Stimulation of cells using EGF-coated magnetic beads.

Stephan J. Kempiak, Jeffrey E. Segall

Research output: Contribution to journalArticle

1 Scopus citations

Abstract

The ability to determine the spatial localization of signals can assist in defining the regulation of specific signaling pathways within the cell. Localized application of a stimulus, followed by imaging of various proteins or their phosphorylated products, allows visualization of the propagation scales of responses to the stimulus. To obtain a localized stimulus of epidermal growth factor (EGF), we bound the ligand to magnetic beads, which could be rapidly pulled onto cell surfaces by a magnet. This method allows the spatial analysis of EGF-induced signaling pathways.

Original languageEnglish (US)
Pages (from-to)pl1
JournalScience's STKE : signal transduction knowledge environment
Volume2004
Issue number218
DOIs
StatePublished - Feb 3 2004

ASJC Scopus subject areas

  • Medicine(all)

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