Steroidogenic factor 1 regulates expression of the cannabinoid receptor 1 in the ventromedial hypothalamic nucleus

Ki Woo Kim, Young-Hwan Jo, Liping Zhao, Nancy R. Stallings, Streamson C. Chua, Jr., Keith L. Parker

Research output: Contribution to journalArticle

24 Citations (Scopus)

Abstract

The nuclear receptor steroidogenic factor 1 (SF-1) plays essential roles in the development and function of the ventromedial hypothalamic nucleus (VMH). Considerable evidence links the VMH and SF-1 with the regulation of energy homeostasis. Here, we demonstrate that SF-1 colocalizes in VMH neurons with the cannabinoid receptor 1 (CB1R) and that a specific CB1R agonist modulates electrical activity of SF-1 neurons in hypothalamic slice preparations. We further show that SF-1 directly regulates CB1R geneexpression via a SF-1-responsive element at -101 in its 5-flanking region. Finally, we show that knockout mice with selective inactivation of SF-1 in the brain have decreased expression of CB1R in the region of the VMH and exhibit a blunted response to systemi-cally administered CB1R agonists. These studies suggest that SF-1 directly regulates the expression of CB1R, which has been implicated in the regulation of energy homeostasis and anxiety-like behavior.

Original languageEnglish (US)
Pages (from-to)1950-1961
Number of pages12
JournalMolecular Endocrinology
Volume22
Issue number8
DOIs
StatePublished - Aug 2008

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Steroidogenic Factor 1
Ventromedial Hypothalamic Nucleus
Cannabinoid Receptors
Homeostasis
Neurons
5' Flanking Region
Cytoplasmic and Nuclear Receptors
Knockout Mice
Anxiety

ASJC Scopus subject areas

  • Molecular Biology
  • Endocrinology, Diabetes and Metabolism

Cite this

Steroidogenic factor 1 regulates expression of the cannabinoid receptor 1 in the ventromedial hypothalamic nucleus. / Kim, Ki Woo; Jo, Young-Hwan; Zhao, Liping; Stallings, Nancy R.; Chua, Jr., Streamson C.; Parker, Keith L.

In: Molecular Endocrinology, Vol. 22, No. 8, 08.2008, p. 1950-1961.

Research output: Contribution to journalArticle

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