Stationary phase induction of dnaN and recF, two genes of Escherichia coli involved in DNA replication and repair

Magda Villarroya, Ignacio Pérez-Roger, Fernando Macian-Juan, M. Eugenia Armengod

Research output: Contribution to journalArticle

30 Citations (Scopus)

Abstract

The β subunit of DNA polymerase III holoenzyme, the Escherichia coli chromosomal replicase, is a sliding DNA clamp responsible for tethering the polymerase to DNA and endowing it with high processivity. The gene encoding β, dnaN, maps between dnnA and recF, which are involved in initiation of DNA replication at oriC and resumption of DNA replication at disrupted replication forks, respectively. In exponentially growing cells, dnaN and recF are expressed predominantly from the dnaA promoters. However, we have found that stationary phase induction of the dnaN promoters drastically changes the expression pattern of the dnaA operon genes. As a striking consequence, synthesis of the β subunit and RecF protein increases when cell metabolism is slowing down. Such an induction is dependent on the stationary phase σ factor, RpoS, although the accumulation of this factor alone is not sufficient to activate the dnaN promoters. These promoters are located in DNA regions without static bending, and the -35 hexamer element is essential for their RpoS-dependent induction. Our results suggest that stationary phase-dependent mechanisms have evolved in order to coordinate expression of dnaN and recF independently of the dnaA regulatory region. These mechanisms might be part of a developmental programme aimed at maintaining DNA integrity under stress conditions.

Original languageEnglish (US)
Pages (from-to)1829-1837
Number of pages9
JournalEMBO Journal
Volume17
Issue number6
DOIs
StatePublished - Mar 16 1998
Externally publishedYes

Fingerprint

DNA Replication
DNA Repair
Escherichia coli
Repair
Genes
DNA
DNA Polymerase III
Holoenzymes
Nucleic Acid Regulatory Sequences
Protein Subunits
DNA-Directed DNA Polymerase
Operon
Gene encoding
Clamping devices
Metabolism
Proteins

Keywords

  • DNA polymerase III holoenzyme
  • DNA replication
  • dnaA-dnaN-recF operon
  • RpoS
  • Stationary phase

ASJC Scopus subject areas

  • Genetics
  • Cell Biology

Cite this

Stationary phase induction of dnaN and recF, two genes of Escherichia coli involved in DNA replication and repair. / Villarroya, Magda; Pérez-Roger, Ignacio; Macian-Juan, Fernando; Armengod, M. Eugenia.

In: EMBO Journal, Vol. 17, No. 6, 16.03.1998, p. 1829-1837.

Research output: Contribution to journalArticle

Villarroya, Magda ; Pérez-Roger, Ignacio ; Macian-Juan, Fernando ; Armengod, M. Eugenia. / Stationary phase induction of dnaN and recF, two genes of Escherichia coli involved in DNA replication and repair. In: EMBO Journal. 1998 ; Vol. 17, No. 6. pp. 1829-1837.
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