STAT3, STAT4, NFATc1, and CTCF regulate PD-1 through multiple novel regulatory regions in murine T cells

James W. Austin, Peiyuan Lu, Parimal Majumder, Rafi Ahmed, Jeremy M. Boss

Research output: Contribution to journalArticle

55 Citations (Scopus)

Abstract

Programmed death-1 (PD-1) is a crucial negative regulator of CD8 T cell development and function, yet the mechanisms that control its expression are not fully understood. Through a nonbiased DNase I hypersensitivity assay, four novel regulatory regions within the Pdcd1 locus were identified. Two of these elements flanked the locus, bound the transcriptional insulator protein CCCTC-binding factor, and interacted with each other, creating a potential regulatory compartmentalization of the locus. In response to T cell activation signaling, NFATc1 bound to two of the novel regions that function as independent regulatory elements. STAT binding sites were identified in these elements as well. In splenic CD8 T cells, TCR-induced PD-1 expression was augmented by IL-6 and IL-12, inducers of STAT3 and STAT4 activity, respectively. IL-6 or IL-12 on its own did not induce PD-1. Importantly, STAT3/4 and distinct chromatin modifications were associated with the novel regulatory regions following cytokine stimulation. The NFATc1/STAT regulatory regions were found to interact with the promoter region of the Pdcd1 gene, providing a mechanism for their action. Together these data add multiple novel distal regulatory regions and pathways to the control of PD-1 expression and provide a molecular mechanism by which proinflammatory cytokines, such as IL-6 or IL-12, can augment PD-1 expression.

Original languageEnglish (US)
Pages (from-to)4876-4886
Number of pages11
JournalJournal of Immunology
Volume192
Issue number10
DOIs
StatePublished - May 15 2014
Externally publishedYes

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Nucleic Acid Regulatory Sequences
T-Lymphocytes
Interleukin-12
Interleukin-6
Cytokines
Deoxyribonuclease I
Genetic Promoter Regions
Protein Binding
Chromatin
Hypersensitivity
Binding Sites
Genes

ASJC Scopus subject areas

  • Immunology

Cite this

STAT3, STAT4, NFATc1, and CTCF regulate PD-1 through multiple novel regulatory regions in murine T cells. / Austin, James W.; Lu, Peiyuan; Majumder, Parimal; Ahmed, Rafi; Boss, Jeremy M.

In: Journal of Immunology, Vol. 192, No. 10, 15.05.2014, p. 4876-4886.

Research output: Contribution to journalArticle

Austin, James W. ; Lu, Peiyuan ; Majumder, Parimal ; Ahmed, Rafi ; Boss, Jeremy M. / STAT3, STAT4, NFATc1, and CTCF regulate PD-1 through multiple novel regulatory regions in murine T cells. In: Journal of Immunology. 2014 ; Vol. 192, No. 10. pp. 4876-4886.
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