Staphylococcus aureus proteins that bind to human endothelial cells

D. C. Tompkins, L. J. Blackwell, Victor Bernard Hatcher, D. A. Elliott, C. O'Hagan-Sotsky, F. D. Lowy

Research output: Contribution to journalArticle

25 Citations (Scopus)

Abstract

The adherence of Staphylococcus aureus to human endothelial cells is saturable in both dose- and time-dependent assays. Staphylococcal surface components which bound to endothelial cells in vitro were identified by using biotin-labeled, solubilized staphylococcal proteins. Four trypsin-sensitive components with molecular sizes of 30, 55 to 57, 70, and 85 kDa were recognized. These proteins did not label with the glycan detection system. When staphylococci were harvested during the exponential phase of growth, staphylococcal adherence to endothelial cells was significantly increased and increased expression of the S. aureus binding proteins was observed. Preincubation of endothelial cells with protein A did not reduce S. aureus adherence in an in vitro infection assay. Four S. aureus surface components whose expression is growth phase dependent adhere to human endothelial cells in vitro.

Original languageEnglish (US)
Pages (from-to)965-969
Number of pages5
JournalInfection and Immunity
Volume60
Issue number3
StatePublished - 1992

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Staphylococcus aureus
Endothelial Cells
Proteins
Staphylococcal Protein A
Growth
Biotin
Staphylococcus
Trypsin
Polysaccharides
Carrier Proteins
Infection
In Vitro Techniques

ASJC Scopus subject areas

  • Immunology

Cite this

Tompkins, D. C., Blackwell, L. J., Hatcher, V. B., Elliott, D. A., O'Hagan-Sotsky, C., & Lowy, F. D. (1992). Staphylococcus aureus proteins that bind to human endothelial cells. Infection and Immunity, 60(3), 965-969.

Staphylococcus aureus proteins that bind to human endothelial cells. / Tompkins, D. C.; Blackwell, L. J.; Hatcher, Victor Bernard; Elliott, D. A.; O'Hagan-Sotsky, C.; Lowy, F. D.

In: Infection and Immunity, Vol. 60, No. 3, 1992, p. 965-969.

Research output: Contribution to journalArticle

Tompkins, DC, Blackwell, LJ, Hatcher, VB, Elliott, DA, O'Hagan-Sotsky, C & Lowy, FD 1992, 'Staphylococcus aureus proteins that bind to human endothelial cells', Infection and Immunity, vol. 60, no. 3, pp. 965-969.
Tompkins DC, Blackwell LJ, Hatcher VB, Elliott DA, O'Hagan-Sotsky C, Lowy FD. Staphylococcus aureus proteins that bind to human endothelial cells. Infection and Immunity. 1992;60(3):965-969.
Tompkins, D. C. ; Blackwell, L. J. ; Hatcher, Victor Bernard ; Elliott, D. A. ; O'Hagan-Sotsky, C. ; Lowy, F. D. / Staphylococcus aureus proteins that bind to human endothelial cells. In: Infection and Immunity. 1992 ; Vol. 60, No. 3. pp. 965-969.
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