Staphylococcus aureus infection of human endothelial cells potentiates Fc receptor expression

Victoria Bengualid, Victor Bernard Hatcher, Betty Diamond, Emily A. Blumberg, Franklin D. Lowy

Research output: Contribution to journalArticle

25 Citations (Scopus)

Abstract

Vasculitis, a recognized complication of staphylococcal-endovascular infections, may result in part, from the expression of FcR by Staphylococcus aureus-infected endothelial cells. FcR were measured using [51]Cr labeled SRBC preincubated with rabbit anti-SRBC IgG. FcR were not detected on uninfected endothelial cells, but were demonstrated on S. aureus infected cells using IgG, but not IgM labeled SRBC. FcR expression was dependent on the initial bacterial density (≥8 × 107 cfu/ml) and on phagocytosis of the staphylococci, but not on new protein synthesis. IgG labeled SRBC binding was blocked by aggregated IgG but not IgM. SRBC coated with the F(ab′)2 portion of IgG did not bind, thus confirming that FcR were specifically involved in this interaction. FcR are expressed after S. aureus invasion of human endothelial cells and may contribute to the vasculitis which often accompanies S. aureus-endovascular infections.

Original languageEnglish (US)
Pages (from-to)4279-4283
Number of pages5
JournalJournal of Immunology
Volume145
Issue number12
StatePublished - Dec 15 1990

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Fc Receptors
Staphylococcus aureus
Endothelial Cells
Immunoglobulin G
Vasculitis
Infection
Immunoglobulin M
Staphylococcal Infections
Staphylococcus
Phagocytosis
Rabbits
Proteins

ASJC Scopus subject areas

  • Immunology

Cite this

Bengualid, V., Hatcher, V. B., Diamond, B., Blumberg, E. A., & Lowy, F. D. (1990). Staphylococcus aureus infection of human endothelial cells potentiates Fc receptor expression. Journal of Immunology, 145(12), 4279-4283.

Staphylococcus aureus infection of human endothelial cells potentiates Fc receptor expression. / Bengualid, Victoria; Hatcher, Victor Bernard; Diamond, Betty; Blumberg, Emily A.; Lowy, Franklin D.

In: Journal of Immunology, Vol. 145, No. 12, 15.12.1990, p. 4279-4283.

Research output: Contribution to journalArticle

Bengualid, V, Hatcher, VB, Diamond, B, Blumberg, EA & Lowy, FD 1990, 'Staphylococcus aureus infection of human endothelial cells potentiates Fc receptor expression', Journal of Immunology, vol. 145, no. 12, pp. 4279-4283.
Bengualid, Victoria ; Hatcher, Victor Bernard ; Diamond, Betty ; Blumberg, Emily A. ; Lowy, Franklin D. / Staphylococcus aureus infection of human endothelial cells potentiates Fc receptor expression. In: Journal of Immunology. 1990 ; Vol. 145, No. 12. pp. 4279-4283.
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