Stage-specific regulation of adhesion molecule expression segregates epithelial stem/progenitor cells in fetal and adult human livers

Mari Inada, Daniel Benten, Kang Cheng, Brigid Joseph, Ekaterine Berishvili, Sunil Badve, Lennart Logdberg, Mariana Dabeva, Sanjeev Gupta

Research output: Contribution to journalArticle

17 Citations (Scopus)

Abstract

Purpose: Regulated expression of cell adhesion molecules could be critical in the proliferation, sequestration, and maintenance of stem/ progenitor cells. Therefore, we determined fetal and adult stage-specific roles of cell adhesion in liver cell compartments. Methods: We performed immunostaining for the adhesion molecules, E-cadherin and Ep-CAM, associated proteins, β-catenin and α-actinin, hepatobiliary markers, albumin, α-fetoprotein, and cytokeratin-19, and the proliferation marker, Ki-67. Expression of albumin was verified by in situ mRNA hybridization. Results: In the fetal liver, hepatoblasts showed extensive proliferation with wide expression of E-cadherin, β-catenin, and α-actinin, although Ep-CAM was expressed in these cells less intensely and focally in the cell membrane to indicate weak cell adhesion. Hepatoblasts in ductal plate and bile ducts showed less proliferation and Ep-CAM was intensely expressed in these cells throughout the cell membrane, indicating strong adhesion. In some ductal plate cells, β-catenin was additionally in the cytoplasm and nucleus, suggesting active cell signaling by adhesion molecules. In adult livers, cells were no longer proliferating and E-cadherin, β-catenin, and α-actinin were expressed in hepatocytes throughout, whereas Ep-CAM was expressed in only bile duct cells. Some cells in ductal structures of the adult liver with Ep-CAM coexpressed albumin and cytokeratin-19, indicating persistence of fetal-like stem/progenitor cells. Conclusions: Regulated expression of Ep-CAM supported proliferation in fetal hepatoblasts through weak adhesion and helped in biliary morphogenesis by promoting stronger adhesion in hepatoblasts during this process. Restriction of Ep-CAM expression to bile ducts in the adult liver presumably facilitated sequestration of stem/progenitor cells. This stage-specific and cell compartment-related regulation of adhesion molecules should be relevant for defining how liver stem/progenitor cells enter, exit, and remain in hepatic niches during both health and disease.

Original languageEnglish (US)
Pages (from-to)50-62
Number of pages13
JournalHepatology International
Volume2
Issue number1
DOIs
StatePublished - Mar 2008

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Stem Cells
Epithelial Cells
Catenins
Liver
Actinin
Cadherins
Bile Ducts
Keratin-19
Albumins
Cell Adhesion Molecules
Cell Adhesion
Cell Membrane
Fetal Proteins
Epithelial Cell Adhesion Molecule
Morphogenesis
In Situ Hybridization
Hepatocytes
Cytoplasm
Maintenance
Messenger RNA

Keywords

  • Adhesion molecules
  • Cell proliferation
  • Stem cells

ASJC Scopus subject areas

  • Hepatology

Cite this

Stage-specific regulation of adhesion molecule expression segregates epithelial stem/progenitor cells in fetal and adult human livers. / Inada, Mari; Benten, Daniel; Cheng, Kang; Joseph, Brigid; Berishvili, Ekaterine; Badve, Sunil; Logdberg, Lennart; Dabeva, Mariana; Gupta, Sanjeev.

In: Hepatology International, Vol. 2, No. 1, 03.2008, p. 50-62.

Research output: Contribution to journalArticle

Inada, Mari ; Benten, Daniel ; Cheng, Kang ; Joseph, Brigid ; Berishvili, Ekaterine ; Badve, Sunil ; Logdberg, Lennart ; Dabeva, Mariana ; Gupta, Sanjeev. / Stage-specific regulation of adhesion molecule expression segregates epithelial stem/progenitor cells in fetal and adult human livers. In: Hepatology International. 2008 ; Vol. 2, No. 1. pp. 50-62.
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T1 - Stage-specific regulation of adhesion molecule expression segregates epithelial stem/progenitor cells in fetal and adult human livers

AU - Inada, Mari

AU - Benten, Daniel

AU - Cheng, Kang

AU - Joseph, Brigid

AU - Berishvili, Ekaterine

AU - Badve, Sunil

AU - Logdberg, Lennart

AU - Dabeva, Mariana

AU - Gupta, Sanjeev

PY - 2008/3

Y1 - 2008/3

N2 - Purpose: Regulated expression of cell adhesion molecules could be critical in the proliferation, sequestration, and maintenance of stem/ progenitor cells. Therefore, we determined fetal and adult stage-specific roles of cell adhesion in liver cell compartments. Methods: We performed immunostaining for the adhesion molecules, E-cadherin and Ep-CAM, associated proteins, β-catenin and α-actinin, hepatobiliary markers, albumin, α-fetoprotein, and cytokeratin-19, and the proliferation marker, Ki-67. Expression of albumin was verified by in situ mRNA hybridization. Results: In the fetal liver, hepatoblasts showed extensive proliferation with wide expression of E-cadherin, β-catenin, and α-actinin, although Ep-CAM was expressed in these cells less intensely and focally in the cell membrane to indicate weak cell adhesion. Hepatoblasts in ductal plate and bile ducts showed less proliferation and Ep-CAM was intensely expressed in these cells throughout the cell membrane, indicating strong adhesion. In some ductal plate cells, β-catenin was additionally in the cytoplasm and nucleus, suggesting active cell signaling by adhesion molecules. In adult livers, cells were no longer proliferating and E-cadherin, β-catenin, and α-actinin were expressed in hepatocytes throughout, whereas Ep-CAM was expressed in only bile duct cells. Some cells in ductal structures of the adult liver with Ep-CAM coexpressed albumin and cytokeratin-19, indicating persistence of fetal-like stem/progenitor cells. Conclusions: Regulated expression of Ep-CAM supported proliferation in fetal hepatoblasts through weak adhesion and helped in biliary morphogenesis by promoting stronger adhesion in hepatoblasts during this process. Restriction of Ep-CAM expression to bile ducts in the adult liver presumably facilitated sequestration of stem/progenitor cells. This stage-specific and cell compartment-related regulation of adhesion molecules should be relevant for defining how liver stem/progenitor cells enter, exit, and remain in hepatic niches during both health and disease.

AB - Purpose: Regulated expression of cell adhesion molecules could be critical in the proliferation, sequestration, and maintenance of stem/ progenitor cells. Therefore, we determined fetal and adult stage-specific roles of cell adhesion in liver cell compartments. Methods: We performed immunostaining for the adhesion molecules, E-cadherin and Ep-CAM, associated proteins, β-catenin and α-actinin, hepatobiliary markers, albumin, α-fetoprotein, and cytokeratin-19, and the proliferation marker, Ki-67. Expression of albumin was verified by in situ mRNA hybridization. Results: In the fetal liver, hepatoblasts showed extensive proliferation with wide expression of E-cadherin, β-catenin, and α-actinin, although Ep-CAM was expressed in these cells less intensely and focally in the cell membrane to indicate weak cell adhesion. Hepatoblasts in ductal plate and bile ducts showed less proliferation and Ep-CAM was intensely expressed in these cells throughout the cell membrane, indicating strong adhesion. In some ductal plate cells, β-catenin was additionally in the cytoplasm and nucleus, suggesting active cell signaling by adhesion molecules. In adult livers, cells were no longer proliferating and E-cadherin, β-catenin, and α-actinin were expressed in hepatocytes throughout, whereas Ep-CAM was expressed in only bile duct cells. Some cells in ductal structures of the adult liver with Ep-CAM coexpressed albumin and cytokeratin-19, indicating persistence of fetal-like stem/progenitor cells. Conclusions: Regulated expression of Ep-CAM supported proliferation in fetal hepatoblasts through weak adhesion and helped in biliary morphogenesis by promoting stronger adhesion in hepatoblasts during this process. Restriction of Ep-CAM expression to bile ducts in the adult liver presumably facilitated sequestration of stem/progenitor cells. This stage-specific and cell compartment-related regulation of adhesion molecules should be relevant for defining how liver stem/progenitor cells enter, exit, and remain in hepatic niches during both health and disease.

KW - Adhesion molecules

KW - Cell proliferation

KW - Stem cells

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