Stable overexpression of MEN1 suppresses tumorigenicity of RAS

Y. S. Kim, A. L. Burns, P. K. Goldsmith, C. Heppner, S. Y. Park, S. C. Chandrasekharappa, F. S. Collins, Allen M. Spiegel, S. J. Marx

Research output: Contribution to journalArticle

93 Citations (Scopus)

Abstract

Although there is indirect genetic evidence that MEN1, the gene for multiple endocrine neoplasia type 1, is a tumor suppressor gene, little is known about the MEN1-encoded protein, menin. Menin was stably overexpressed in a well-characterized murine tumor cell line, (valine-12)-RAS-transformed NIH3T3 cells. Menin overexpression reverted the morphology of the RAS-transformed NIH3T3 cells towards the more flattened and more spread, fibroblastic shape of wild type NIH3T3 cells. The proliferation rate of the RAS-transformed cells in 0.5% calf serum was also slower with menin overexpression. Menin overexpression reduced the RAS-induced clonogenicity in soft agar. Menin also reduced tumor growth after injection of cells in nude mice. In conclusion, stable overexpression of MEN1 suppressed partially the RAS-mediated tumor phenotype in vitro and in vivo. Overexpressed menin protein had biological effects, directly supporting MEN1 gene function as a tumor suppressor.

Original languageEnglish (US)
Pages (from-to)5936-5942
Number of pages7
JournalOncogene
Volume18
Issue number43
DOIs
StatePublished - Oct 21 1999
Externally publishedYes

Fingerprint

Multiple Endocrine Neoplasia Type 1
Neoplasms
Valine
Tumor Cell Line
Tumor Suppressor Genes
Nude Mice
Genes
Agar
Proteins
Phenotype
Injections
Growth
Serum

Keywords

  • Menin, MEN1
  • Neoplasia
  • NIH3T3
  • Oncogene
  • RAS
  • Tumor suppressor

ASJC Scopus subject areas

  • Molecular Biology
  • Cancer Research
  • Genetics

Cite this

Kim, Y. S., Burns, A. L., Goldsmith, P. K., Heppner, C., Park, S. Y., Chandrasekharappa, S. C., ... Marx, S. J. (1999). Stable overexpression of MEN1 suppresses tumorigenicity of RAS. Oncogene, 18(43), 5936-5942. https://doi.org/10.1038/sj.onc.1203005

Stable overexpression of MEN1 suppresses tumorigenicity of RAS. / Kim, Y. S.; Burns, A. L.; Goldsmith, P. K.; Heppner, C.; Park, S. Y.; Chandrasekharappa, S. C.; Collins, F. S.; Spiegel, Allen M.; Marx, S. J.

In: Oncogene, Vol. 18, No. 43, 21.10.1999, p. 5936-5942.

Research output: Contribution to journalArticle

Kim, YS, Burns, AL, Goldsmith, PK, Heppner, C, Park, SY, Chandrasekharappa, SC, Collins, FS, Spiegel, AM & Marx, SJ 1999, 'Stable overexpression of MEN1 suppresses tumorigenicity of RAS', Oncogene, vol. 18, no. 43, pp. 5936-5942. https://doi.org/10.1038/sj.onc.1203005
Kim YS, Burns AL, Goldsmith PK, Heppner C, Park SY, Chandrasekharappa SC et al. Stable overexpression of MEN1 suppresses tumorigenicity of RAS. Oncogene. 1999 Oct 21;18(43):5936-5942. https://doi.org/10.1038/sj.onc.1203005
Kim, Y. S. ; Burns, A. L. ; Goldsmith, P. K. ; Heppner, C. ; Park, S. Y. ; Chandrasekharappa, S. C. ; Collins, F. S. ; Spiegel, Allen M. ; Marx, S. J. / Stable overexpression of MEN1 suppresses tumorigenicity of RAS. In: Oncogene. 1999 ; Vol. 18, No. 43. pp. 5936-5942.
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