Stable lentiviral vector-mediated gene silencing in human monocytic cell lines

Jimmy S. Lee, Neil E. Reiner

Research output: Chapter in Book/Report/Conference proceedingChapter

2 Citations (Scopus)

Abstract

A major obstacle in studying monocyte cell biology has been the resistance of these cells to genetic manipulation, particularly when using nonviral methods. In the approach outlined in this chapter, we describe a strategy for stable gene silencing of monocytic cells. Using a vesicular stomatitis virus (VSV)-pseudotyped lentiviral vector expressing short hairpin RNA (shRNA), stable silencing of the p110α isoform of PI3-kinase in the human monocytic cell lines THP-1 and U-937 was achieved.

Original languageEnglish (US)
Title of host publicationMacrophages and Dendritic Cells
Subtitle of host publicationMethods and Protocols
Pages287-300
Number of pages14
Volume531
DOIs
StatePublished - 2009
Externally publishedYes

Publication series

NameMethods in Molecular Biology
Volume531
ISSN (Print)1064-3745

Fingerprint

Gene Silencing
Cell Line
Vesicular Stomatitis
RNA Interference
Phosphatidylinositol 3-Kinases
Small Interfering RNA
Cell Biology
Monocytes
Protein Isoforms
Viruses

Keywords

  • Lentiviral vector
  • Monocyte
  • PI3-kinase
  • RNAi
  • shRNA
  • siRNA
  • Transduction

ASJC Scopus subject areas

  • Molecular Biology
  • Genetics

Cite this

Lee, J. S., & Reiner, N. E. (2009). Stable lentiviral vector-mediated gene silencing in human monocytic cell lines. In Macrophages and Dendritic Cells: Methods and Protocols (Vol. 531, pp. 287-300). (Methods in Molecular Biology; Vol. 531). https://doi.org/10.1007/978-1-59745-396-7_18

Stable lentiviral vector-mediated gene silencing in human monocytic cell lines. / Lee, Jimmy S.; Reiner, Neil E.

Macrophages and Dendritic Cells: Methods and Protocols. Vol. 531 2009. p. 287-300 (Methods in Molecular Biology; Vol. 531).

Research output: Chapter in Book/Report/Conference proceedingChapter

Lee, JS & Reiner, NE 2009, Stable lentiviral vector-mediated gene silencing in human monocytic cell lines. in Macrophages and Dendritic Cells: Methods and Protocols. vol. 531, Methods in Molecular Biology, vol. 531, pp. 287-300. https://doi.org/10.1007/978-1-59745-396-7_18
Lee JS, Reiner NE. Stable lentiviral vector-mediated gene silencing in human monocytic cell lines. In Macrophages and Dendritic Cells: Methods and Protocols. Vol. 531. 2009. p. 287-300. (Methods in Molecular Biology). https://doi.org/10.1007/978-1-59745-396-7_18
Lee, Jimmy S. ; Reiner, Neil E. / Stable lentiviral vector-mediated gene silencing in human monocytic cell lines. Macrophages and Dendritic Cells: Methods and Protocols. Vol. 531 2009. pp. 287-300 (Methods in Molecular Biology).
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