Stable lentiviral vector-mediated gene silencing in human monocytic cell lines

Jimmy S. Lee, Neil E. Reiner

Research output: Chapter in Book/Report/Conference proceedingChapter

3 Scopus citations

Abstract

A major obstacle in studying monocyte cell biology has been the resistance of these cells to genetic manipulation, particularly when using nonviral methods. In the approach outlined in this chapter, we describe a strategy for stable gene silencing of monocytic cells. Using a vesicular stomatitis virus (VSV)-pseudotyped lentiviral vector expressing short hairpin RNA (shRNA), stable silencing of the p110α isoform of PI3-kinase in the human monocytic cell lines THP-1 and U-937 was achieved.

Original languageEnglish (US)
Title of host publicationMacrophages and Dendritic Cells
Subtitle of host publicationMethods and Protocols
EditorsNeil Reiner
Pages287-300
Number of pages14
DOIs
StatePublished - Dec 1 2009
Externally publishedYes

Publication series

NameMethods in Molecular Biology
Volume531
ISSN (Print)1064-3745

Keywords

  • Lentiviral vector
  • Monocyte
  • PI3-kinase
  • RNAi
  • Transduction
  • shRNA
  • siRNA

ASJC Scopus subject areas

  • Molecular Biology
  • Genetics

Fingerprint Dive into the research topics of 'Stable lentiviral vector-mediated gene silencing in human monocytic cell lines'. Together they form a unique fingerprint.

  • Cite this

    Lee, J. S., & Reiner, N. E. (2009). Stable lentiviral vector-mediated gene silencing in human monocytic cell lines. In N. Reiner (Ed.), Macrophages and Dendritic Cells: Methods and Protocols (pp. 287-300). (Methods in Molecular Biology; Vol. 531). https://doi.org/10.1007/978-1-59745-396-7_18