STABLE ISOTOPE DILUTION THERMOSPRAY LIQUID CHROMATOGRAPHY/MASS SPECTROMETRY METHOD FOR DETERMINATION OF SUGARS AND SUGAR ALCOHOLS IN HUMANS.

Nora V. Esteban, Daniel J. Liberato, James B. Sidbury, Alfred L. Yergey

Research output: Contribution to journalArticle

28 Citations (Scopus)

Abstract

The development of a thermospray high-performance liquid chromatography/mass spectrometry method for the determination of carbohydrates and sugar alcohols is reported. The simultaneous separation and identificiation of mono- and disaccharides as well as sugar alcohols has a detection limit of about 50 pmol of material on column. Isotope dilution mass spectrometry (IDMS) allows determinations of glucose at low isotopic enrichments (0. 1-1%) and sorbitol at approximately 1:l labeled/unlabeled ratio, in small serum or amniotic fluid samples (200 mu L), respectively. Application of the IDMS method to the determination of sorbitol concentration in amniotic fluid and in a child with glycogen storage disease type I confirmed the validity of this technique for clinical investigation.

Original languageEnglish (US)
Pages (from-to)1674-1677
Number of pages4
JournalAnalytical Chemistry
Volume59
Issue number13
StatePublished - Jul 1 1987
Externally publishedYes

Fingerprint

Sugar Alcohols
Liquid chromatography
Sugars
Isotopes
Dilution
Mass spectrometry
Sorbitol
Fluids
Monosaccharides
Disaccharides
High performance liquid chromatography
Glycogen
Carbohydrates
Glucose

ASJC Scopus subject areas

  • Analytical Chemistry

Cite this

STABLE ISOTOPE DILUTION THERMOSPRAY LIQUID CHROMATOGRAPHY/MASS SPECTROMETRY METHOD FOR DETERMINATION OF SUGARS AND SUGAR ALCOHOLS IN HUMANS. / Esteban, Nora V.; Liberato, Daniel J.; Sidbury, James B.; Yergey, Alfred L.

In: Analytical Chemistry, Vol. 59, No. 13, 01.07.1987, p. 1674-1677.

Research output: Contribution to journalArticle

Esteban, Nora V. ; Liberato, Daniel J. ; Sidbury, James B. ; Yergey, Alfred L. / STABLE ISOTOPE DILUTION THERMOSPRAY LIQUID CHROMATOGRAPHY/MASS SPECTROMETRY METHOD FOR DETERMINATION OF SUGARS AND SUGAR ALCOHOLS IN HUMANS. In: Analytical Chemistry. 1987 ; Vol. 59, No. 13. pp. 1674-1677.
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