Stable Isotope Dilution Thermospray Liquid Chromatography/Mass Spectrometry Method for Determination of Sugars and Sugar Alcohols in Humans

Nora V. Esteban; Daniel J. Liberato; James B. Sidbury; Alfred L. Yergey

doi:10.1021/ac00140a020

Stable Isotope Dilution Thermospray Liquid Chromatography/Mass Spectrometry Method for Determination of Sugars and Sugar Alcohols in Humans

Nora V. Esteban, Daniel J. Liberato, James B. Sidbury, Alfred L. Yergey

Pediatrics

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Abstract

The development of a thermospray high-performance liquid chromatography/mass spectrometry method for the determination of carbohydrates and sugar alcohols is reported. The simultaneous separation and identification of mono- and disaccharides as well as sugar alcohols has a detection limit of about 50 pmol of material on column. Isotope dilution mass spectrometry (IDMS) allows determinations of glucose at low isotopic enrichments (0.1-1%) and sorbitol at ~1:1 labeled/unlabeled ratio, in small serum or amniotic fluid samples (200 μL), respectively. Application of the IDMS method to the determination of sorbitol concentration in amniotic fluid and in a child with glycogen storage disease type I confirmed the validity of this technique for clinical investigation.

Original language	English (US)
Pages (from-to)	1674-1677
Number of pages	4
Journal	Analytical Chemistry
Volume	59
Issue number	13
DOIs	https://doi.org/10.1021/ac00140a020
State	Published - Jan 1 1987

ASJC Scopus subject areas

Analytical Chemistry

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title = "Stable Isotope Dilution Thermospray Liquid Chromatography/Mass Spectrometry Method for Determination of Sugars and Sugar Alcohols in Humans",

abstract = "The development of a thermospray high-performance liquid chromatography/mass spectrometry method for the determination of carbohydrates and sugar alcohols is reported. The simultaneous separation and identification of mono- and disaccharides as well as sugar alcohols has a detection limit of about 50 pmol of material on column. Isotope dilution mass spectrometry (IDMS) allows determinations of glucose at low isotopic enrichments (0.1-1%) and sorbitol at ~1:1 labeled/unlabeled ratio, in small serum or amniotic fluid samples (200 μL), respectively. Application of the IDMS method to the determination of sorbitol concentration in amniotic fluid and in a child with glycogen storage disease type I confirmed the validity of this technique for clinical investigation.",

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AU - Yergey, Alfred L.

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AB - The development of a thermospray high-performance liquid chromatography/mass spectrometry method for the determination of carbohydrates and sugar alcohols is reported. The simultaneous separation and identification of mono- and disaccharides as well as sugar alcohols has a detection limit of about 50 pmol of material on column. Isotope dilution mass spectrometry (IDMS) allows determinations of glucose at low isotopic enrichments (0.1-1%) and sorbitol at ~1:1 labeled/unlabeled ratio, in small serum or amniotic fluid samples (200 μL), respectively. Application of the IDMS method to the determination of sorbitol concentration in amniotic fluid and in a child with glycogen storage disease type I confirmed the validity of this technique for clinical investigation.

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Stable Isotope Dilution Thermospray Liquid Chromatography/Mass Spectrometry Method for Determination of Sugars and Sugar Alcohols in Humans

Abstract

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