The development of a thermospray high-performance liquid chromatography/mass spectrometry method for the determination of carbohydrates and sugar alcohols is reported. The simultaneous separation and identification of mono- and disaccharides as well as sugar alcohols has a detection limit of about 50 pmol of material on column. Isotope dilution mass spectrometry (IDMS) allows determinations of glucose at low isotopic enrichments (0.1-1%) and sorbitol at ~1:1 labeled/unlabeled ratio, in small serum or amniotic fluid samples (200 μL), respectively. Application of the IDMS method to the determination of sorbitol concentration in amniotic fluid and in a child with glycogen storage disease type I confirmed the validity of this technique for clinical investigation.
ASJC Scopus subject areas
- Analytical Chemistry