Stable expression of mosaic coats of variant surface glycoproteins in Trypanosoma brucei

Jorge L. Muñoz-Jordán, Kelvin Davies, George A M Cross

Research output: Contribution to journalArticle

45 Citations (Scopus)

Abstract

The paradigm of antigenic variation in parasites is the variant surface glycoprotein (VSG) of African trypanosomes. Only one VSG is expressed at any time, except for short periods during switching. The reasons for this pattern of expression and the consequences of expressing more than one VSG are unknown. Trypanosoma brucei was genetically manipulated to generate cell lines that expressed two VSGs simultaneously. These VSGs were produced in equal amounts and were homogeneously distributed on the trypanosome surface. The double-expressor cells had similar population doubling times and were as infective as wild-type cells. Thus, the simultaneous expression of two VSGs is not intrinsically harmful.

Original languageEnglish (US)
Pages (from-to)1795-1797
Number of pages3
JournalScience
Volume272
Issue number5269
StatePublished - Jun 21 1996
Externally publishedYes

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Trypanosoma brucei brucei
Membrane Glycoproteins
Trypanosomiasis
Antigenic Variation
Parasites
Cell Line
Population

ASJC Scopus subject areas

  • General

Cite this

Muñoz-Jordán, J. L., Davies, K., & Cross, G. A. M. (1996). Stable expression of mosaic coats of variant surface glycoproteins in Trypanosoma brucei. Science, 272(5269), 1795-1797.

Stable expression of mosaic coats of variant surface glycoproteins in Trypanosoma brucei. / Muñoz-Jordán, Jorge L.; Davies, Kelvin; Cross, George A M.

In: Science, Vol. 272, No. 5269, 21.06.1996, p. 1795-1797.

Research output: Contribution to journalArticle

Muñoz-Jordán, JL, Davies, K & Cross, GAM 1996, 'Stable expression of mosaic coats of variant surface glycoproteins in Trypanosoma brucei', Science, vol. 272, no. 5269, pp. 1795-1797.
Muñoz-Jordán, Jorge L. ; Davies, Kelvin ; Cross, George A M. / Stable expression of mosaic coats of variant surface glycoproteins in Trypanosoma brucei. In: Science. 1996 ; Vol. 272, No. 5269. pp. 1795-1797.
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