Stability of polyadenylated RNA in differentiating myogenic cells

Robert H. Singer, G. Kessler-Icekson

Research output: Contribution to journalArticle

14 Citations (Scopus)

Abstract

Three independent methods of measurement showed that cytoplasmic polyadenylated RNA from the differentiating myogenic cell line L8 consists of two main populations with regard to stability, one with a half-life of less than 4 h and the other with a half-life of 17-54 h. Similar results were obtained in the presence and absence of actinomycin D. During the fusion of mononucleated myoblasts into multinucleated fibers, there was an increase in both the steady-state pool of the more stable polyadenylated RNA and the proportion of stable polyadenylated RNA synthesized in pulse labelling.

Original languageEnglish (US)
Pages (from-to)395-402
Number of pages8
JournalEuropean Journal of Biochemistry
Volume88
Issue number2
StatePublished - 1978
Externally publishedYes

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Messenger RNA
Half-Life
Myoblasts
Dactinomycin
Labeling
Fusion reactions
Cells
Cell Line
Fibers
Population

ASJC Scopus subject areas

  • Biochemistry

Cite this

Stability of polyadenylated RNA in differentiating myogenic cells. / Singer, Robert H.; Kessler-Icekson, G.

In: European Journal of Biochemistry, Vol. 88, No. 2, 1978, p. 395-402.

Research output: Contribution to journalArticle

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