Spontaneous Intracellular Calcium Oscillations and Gsα Subunit Expression are Inversely Correlated with Secretory Granule Content in Pituitary Cells

Carlo Chiavaroli, Dermot M.F. Cooper, Christine L. Boyajian, Rosemary Murray‐Whelan, Nicolas Demaurex, Allen M. Spiegel, Werner Schlegel

Research output: Contribution to journalArticle

3 Scopus citations

Abstract

Cells of the pituitary tumour cell line GH4C1 were exposed to epidermal growth factor, estradiol and insulin for 5 days, a treatment which resulted in 1) increased prolactin storage in secretory granules, 2) the loss of spontaneous [Ca2+]1 oscillations, and 3) a selective reduction of the protein Gsα, seen in immunoblots, cholera toxin labelling, and vasoactive intestinal peptide stimulation of adenylyl cyclase. In contrast, the glucocorticoid dexamethasone, which increases the expression of Gsα, partially restored spontaneous [Ca2+]1 oscillations and decreased prolactin storage. It is concluded that Gsα levels in tumoral cells result in spontaneous electrical activity which may empty prolactin stores by the continuous activation of exocytosis.

Original languageEnglish (US)
Pages (from-to)473-481
Number of pages9
JournalJournal of Neuroendocrinology
Volume4
Issue number4
DOIs
StatePublished - Aug 1992
Externally publishedYes

Keywords

  • GHC cells
  • G‐proteins
  • [Ca]
  • oscillations
  • secretory granules

ASJC Scopus subject areas

  • Endocrinology, Diabetes and Metabolism
  • Endocrinology
  • Endocrine and Autonomic Systems
  • Cellular and Molecular Neuroscience

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