Spontaneous and X-ray-induced deletion mutations in a LacZ plasmid-based transgenic mouse model

Jan A. Gossen, Hans Jörg Martus, Jeanne Y. Wei, Jan Vijg

Research output: Contribution to journalArticle

68 Scopus citations

Abstract

Transgenic mouse mutation models carrying bacterial marker genes in bacteriophage lambda shuttle vectors have been applied to study spontaneous or induced mutations in vivo. However, due to the nature of the shuttle vector these models are insensitive to large deletions. Clastogenic agents, which predominantly induce large deletions, were therefore found to yeild very low responses in these assays. Here we report the use of LacZ plasmid-based transgenic mice, allowing the detection of a broad spectrum of mutations. Treatment of mice with X-rays (5 × 50 rads) resulted in induction of up to about 5-fold higher mutation frequencies in lung, spleen and liver. Analysis of spontaneous and induced mutant LacZ genes indicated that at least 40-50% of all mutations were caused by deletions. The possibility of detecting a broad spectrum of mutations with this system suggests that the LacZ plasmid-based transgenic mouse may be the mammalian model of choice for studying spontaneous and induced mutations in vivo.

Original languageEnglish (US)
Pages (from-to)89-97
Number of pages9
JournalMutation Research - Fundamental and Molecular Mechanisms of Mutagenesis
Volume331
Issue number1
DOIs
Publication statusPublished - Sep 1995
Externally publishedYes

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Keywords

  • Deletions
  • LacZ mutant selection
  • Plasmid rescue
  • Transgenic mice
  • X-rays

ASJC Scopus subject areas

  • Molecular Biology
  • Genetics
  • Health, Toxicology and Mutagenesis

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