Spine expansion and stabilization associated with long-term potentiation

Yunlei Yang, Xiao Bin Wang, Matthew Frerking, Qiang Zhou

Research output: Contribution to journalArticle

136 Citations (Scopus)

Abstract

Stable expression of long-term synaptic plasticity is critical for the developmental refinement of neural circuits and for some forms of learning and memory. Although structural remodeling of dendritic spines is associated with the stable expression of long-term potentiation (LTP), the relationship between structural and physiological plasticity remains unclear. To define whether these two processes are related or distinct, we simultaneously monitored EPSPs and dendritic spines, using combined patch-clamp recording and two-photon time-lapse imaging in the same CA1 pyramidal neurons in acute hippocampal slices. We found that theta burst stimulation paired with postsynaptic spiking, which reliably induced LTP, also induced a rapid and persistent expansion of dendritic spines. Like LTP, this expansion was NMDA receptor dependent. Spine expansion occurred even when LTP was inhibited by postsynaptic inhibition of exocytosis or PKA (protein kinase A); however, under these conditions, the spine expansion was unstable and collapsed spontaneously. Furthermore, similar changes in LTP and spine expansion were observed when hippocampal neurons were treated with protein synthesis inhibitors. Like LTP, spine expansion was reversed by low-frequency stimulation (LFS) via a phosphatase-dependent mechanism, but only if the LFS was applied in a critical time window after induction. These results indicate that the initial expression of LTP and spine expansion is dissociable, but there is a high degree of mechanistic overlap between the stabilization of structural plasticity and LTP.

Original languageEnglish (US)
Pages (from-to)5740-5751
Number of pages12
JournalJournal of Neuroscience
Volume28
Issue number22
DOIs
StatePublished - May 28 2008
Externally publishedYes

Fingerprint

Long-Term Potentiation
Spine
Dendritic Spines
Time-Lapse Imaging
Neuronal Plasticity
Protein Synthesis Inhibitors
Pyramidal Cells
Excitatory Postsynaptic Potentials
Exocytosis
Cyclic AMP-Dependent Protein Kinases
N-Methyl-D-Aspartate Receptors
Photons
Phosphoric Monoester Hydrolases
Learning
Neurons

Keywords

  • Actin
  • Ampa receptors
  • Dendritic spines
  • Depotentiation
  • LTP
  • Two-photon imaging

ASJC Scopus subject areas

  • Neuroscience(all)

Cite this

Spine expansion and stabilization associated with long-term potentiation. / Yang, Yunlei; Wang, Xiao Bin; Frerking, Matthew; Zhou, Qiang.

In: Journal of Neuroscience, Vol. 28, No. 22, 28.05.2008, p. 5740-5751.

Research output: Contribution to journalArticle

Yang, Yunlei ; Wang, Xiao Bin ; Frerking, Matthew ; Zhou, Qiang. / Spine expansion and stabilization associated with long-term potentiation. In: Journal of Neuroscience. 2008 ; Vol. 28, No. 22. pp. 5740-5751.
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