Spindle-localized CPE-mediated translation controls meiotic chromosome segregation

Carolina Eliscovich, Isabel Peset, Isabelle Vernos, Raúl Méndez

Research output: Contribution to journalArticle

56 Citations (Scopus)

Abstract

Meiotic progression requires the translational activation of stored maternal mRNAs, such as those encoding cyclin B1 or mos. The translation of these mRNAs is regulated by the cytoplasmic polyadenylation element (CPE) present in their 3′UTRs, which recruits the CPE-binding protein CPEB. This RNA-binding protein not only dictates the timing and extent of translational activation by cytoplasmic polyadenylation but also participates, together with the translational repressor Maskin, in the transport and localization, in a quiescent state, of its targets to subcellular locations where their translation will take place. During the early development of Xenopus laevis, CPEB localizes at the animal pole of oocytes and later on at embryonic spindles and centrosomes. Disruption of embryonic CPEB-mediated translational regulation results in abnormalities in the mitotic apparatus and inhibits embryonic mitosis. Here we show that spindle-localized translational activation of CPE-regulated mRNAs, encoding for proteins with a known function in spindle assembly and chromosome segregation, is essential for completion of the first meiotic division and for chromosome segregation in Xenopus oocytes.

Original languageEnglish (US)
Pages (from-to)858-865
Number of pages8
JournalNature Cell Biology
Volume10
Issue number7
DOIs
StatePublished - Jul 2008
Externally publishedYes

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Chromosome Segregation
Polyadenylation
Stored Messenger RNA
Oocytes
Cyclin B1
Centrosome
Spindle Apparatus
RNA-Binding Proteins
Xenopus laevis
Protein Biosynthesis
Xenopus
Mitosis
Carrier Proteins
Messenger RNA
Proteins

ASJC Scopus subject areas

  • Cell Biology

Cite this

Spindle-localized CPE-mediated translation controls meiotic chromosome segregation. / Eliscovich, Carolina; Peset, Isabel; Vernos, Isabelle; Méndez, Raúl.

In: Nature Cell Biology, Vol. 10, No. 7, 07.2008, p. 858-865.

Research output: Contribution to journalArticle

Eliscovich, Carolina ; Peset, Isabel ; Vernos, Isabelle ; Méndez, Raúl. / Spindle-localized CPE-mediated translation controls meiotic chromosome segregation. In: Nature Cell Biology. 2008 ; Vol. 10, No. 7. pp. 858-865.
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