Spectroscopic determination of chelator content in antibody-chelator conjugates: UV/VIS, fluorescence, and MALDI-TOF MS studies

Ekaterina Dadachova

Research output: Contribution to journalArticle

Abstract

The increasing use of monoclonal antibody-bifunctional chelator conjugates in clinical practice has created the need for quality control procedures providing relatively simple and reliable determination of the chelator-to-protein (C/P) ratio. Spectroscopic methods offer a valuable alternative to the use of radioactive isotopes for this purpose. This article discusses the application of UW/Vis spectrophotometry, fluorescence spectroscopy, and matrix-assisted laser desorption ionization time-of-flight mass spectrometry (MALDI-TOF MS) for the quality control of MAb-chelator conjugates.

Original languageEnglish (US)
Pages (from-to)30-32
Number of pages3
JournalSpectroscopy (Santa Monica)
Volume15
Issue number1
StatePublished - Dec 1 2000

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ASJC Scopus subject areas

  • Analytical Chemistry
  • Atomic and Molecular Physics, and Optics
  • Spectroscopy

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