Spectroscopic determination of chelator content in antibody-chelator conjugates: UV/VIS, fluorescence, and MALDI-TOF MS studies

Ekaterina Dadachova

Research output: Contribution to journalArticle

Abstract

The increasing use of monoclonal antibody-bifunctional chelator conjugates in clinical practice has created the need for quality control procedures providing relatively simple and reliable determination of the chelator-to-protein (C/P) ratio. Spectroscopic methods offer a valuable alternative to the use of radioactive isotopes for this purpose. This article discusses the application of UW/Vis spectrophotometry, fluorescence spectroscopy, and matrix-assisted laser desorption ionization time-of-flight mass spectrometry (MALDI-TOF MS) for the quality control of MAb-chelator conjugates.

Original languageEnglish (US)
Pages (from-to)30-32
Number of pages3
JournalSpectroscopy (Santa Monica)
Volume15
Issue number1
StatePublished - 2000
Externally publishedYes

Fingerprint

Chelating Agents
quality control
antibodies
Ionization
Mass spectrometry
Desorption
mass spectroscopy
desorption
Fluorescence
ionization
fluorescence
Quality control
Antibodies
Lasers
spectrophotometry
matrices
radioactive isotopes
lasers
Fluorescence spectroscopy
Spectrophotometry

ASJC Scopus subject areas

  • Spectroscopy

Cite this

Spectroscopic determination of chelator content in antibody-chelator conjugates : UV/VIS, fluorescence, and MALDI-TOF MS studies. / Dadachova, Ekaterina.

In: Spectroscopy (Santa Monica), Vol. 15, No. 1, 2000, p. 30-32.

Research output: Contribution to journalArticle

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