Specificity of RNA chain initiation by bacteriophage T7-induced RNA polymerase

Subal Bishayee, Arturo Yudelevich, Umadas Maitra

Research output: Contribution to journalArticle

1 Scopus citations

Abstract

Analysis of the nucleotide sequence at the 5′-triphosphate termini of RNA chains synthesized by T7 RNA polymerase from T7 DNA template indicates that nearly all RNA chains synthesized in this polymerase reaction contain the sequence, pppGpGp. In addition, studies carried out on T7 DNA-dependent 32PPi exchange into ribonucleoside triphosphates suggest that immediately following the guanine residues at the 5′-end of RNA formed in the T7 RNA polymerase reaction, there is one or more adenine residues. These results indicate a high degree of specificity of initiation of RNA synthesis by T7 RNA polymerase.

Original languageEnglish (US)
Pages (from-to)626-633
Number of pages8
JournalBiochemical and Biophysical Research Communications
Volume68
Issue number2
DOIs
StatePublished - Jan 26 1976

ASJC Scopus subject areas

  • Biophysics
  • Biochemistry
  • Molecular Biology
  • Cell Biology

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