Specificity of ribonucleic acid chain initiation by bacteriophage T3 induced ribonucleic acid polymerase

U. Maitra, R. A. Salvo, P. R. Chakraborty

Research output: Contribution to journalArticle

8 Citations (Scopus)

Abstract

Further studies on the specificity of RNA chain initiation by T3 induced RNA polymerase were carried out. The nucleotide penultimate to the 5' guanosine triphosphate end of RNA chains was determined. GMP is found exclusively in this second position of a growing RNA chain. The 32PP(i) exchange reaction with ribonucleoside triphosphates catalyzed by T3 RNA polymerase on native T3 DNA template was also studied as a measure of the initiation reaction. It has been found that T3 RNA polymerase carries out an active exchange of 32PP(i) into ATP in the presence of GTP. In contrast, in reactions containing GTP alone or with GTP + CTP or GTP + UTP, an active exchange of 32PP(i) only occurs into GTP. No 32PP(i) incorporation was detected into either CTP or UTP. These results suggest that all RNA chains formed in T3 RNA polymerase reaction directed by native T3 DNA as template contain the sequence pppGp(Gp)n(Ap)m... (where n and m = 1 or more) at the 5' terminus, indicating a high degree of specificity of initiation of RNA chains in this system.

Original languageEnglish (US)
Pages (from-to)5835-5839
Number of pages5
JournalJournal of Biological Chemistry
Volume249
Issue number18
StatePublished - 1974

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Bacteriophage T3
Bacteriophages
Guanosine Triphosphate
RNA
Cytidine Triphosphate
Uridine Triphosphate
Ribonucleosides
DNA
DNA-Directed RNA Polymerases
Nucleotides
Adenosine Triphosphate
bacteriophage T3 RNA polymerase

ASJC Scopus subject areas

  • Biochemistry

Cite this

Specificity of ribonucleic acid chain initiation by bacteriophage T3 induced ribonucleic acid polymerase. / Maitra, U.; Salvo, R. A.; Chakraborty, P. R.

In: Journal of Biological Chemistry, Vol. 249, No. 18, 1974, p. 5835-5839.

Research output: Contribution to journalArticle

Maitra, U. ; Salvo, R. A. ; Chakraborty, P. R. / Specificity of ribonucleic acid chain initiation by bacteriophage T3 induced ribonucleic acid polymerase. In: Journal of Biological Chemistry. 1974 ; Vol. 249, No. 18. pp. 5835-5839.
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