TY - JOUR
T1 - Specific identification of Mycobacterium tuberculosis with the luciferase reporter mycobacteriophage
T2 - Use of p-nitro-α-acetylamino-β- hydroxy propiophenone
AU - Riska, Paul F.
AU - Jacobs, William R.
AU - Bloom, Barry R.
AU - Mckitrick, John
AU - Chan, John
PY - 1997/12
Y1 - 1997/12
N2 - We have previously described a luciferase reporter mycobacteriophage (LRP) assay that can detect Mycobacterium tuberculosis and characterize mycobacterial drug susceptibility patterns within 24 to 48 h in positive cultures. One drawback of this LRP protocol is the ability of the recombinant mycobacteriophage phAE40 to infect a variety of Mycobacterium species, thus limiting its specificity for the detection of M. tuberculosis. In this study, we have (i) explored the host range of phAE40, (ii) developed a modified LRP assay that exploits the selective inhibitory effect of the Compound p-nitro- α-acetylamino-β-hydroxy propiophenone (NAP) against members of the M. tuberculosis complex to differentiate between the tubercle bacillus and other mycobacterial species, and (iii) tested over 300 samples, including primary clinical isolates and drug-resistant strains of M. tuberculosis, demonstrating the ability of the NAP-modified LRP assay to identify M. tuberculosis complex organisms with high degrees of sensitivity and specificity.
AB - We have previously described a luciferase reporter mycobacteriophage (LRP) assay that can detect Mycobacterium tuberculosis and characterize mycobacterial drug susceptibility patterns within 24 to 48 h in positive cultures. One drawback of this LRP protocol is the ability of the recombinant mycobacteriophage phAE40 to infect a variety of Mycobacterium species, thus limiting its specificity for the detection of M. tuberculosis. In this study, we have (i) explored the host range of phAE40, (ii) developed a modified LRP assay that exploits the selective inhibitory effect of the Compound p-nitro- α-acetylamino-β-hydroxy propiophenone (NAP) against members of the M. tuberculosis complex to differentiate between the tubercle bacillus and other mycobacterial species, and (iii) tested over 300 samples, including primary clinical isolates and drug-resistant strains of M. tuberculosis, demonstrating the ability of the NAP-modified LRP assay to identify M. tuberculosis complex organisms with high degrees of sensitivity and specificity.
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U2 - 10.1128/jcm.35.12.3225-3231.1997
DO - 10.1128/jcm.35.12.3225-3231.1997
M3 - Article
C2 - 9399524
AN - SCOPUS:0030724353
SN - 0095-1137
VL - 35
SP - 3225
EP - 3231
JO - Journal of Clinical Microbiology
JF - Journal of Clinical Microbiology
IS - 12
ER -