Specific growth rate determines the sensitivity of Escherichia coli to thermal, UVA, and solar disinfection

Michael Berney, Hans Ulrich Weilenmann, Julian Ihssen, Claudio Bassin, Thomas Egli

Research output: Contribution to journalArticle

149 Citations (Scopus)

Abstract

Knowledge about the sensitivity of the test organism is essential for the evaluation of any disinfection method. In this work we show that sensitivity of Escherichia coli MG1655 to three physical stresses (mild heat, UVA light, and sunlight) that are relevant in the disinfection of drinking water with solar radiation is determined by the specific growth rate of the culture. Batch- and chemostat-cultivated cells from cultures with similar specific growth rates showed similar stress sensitivities. Generally, fast-growing cells were more sensitive to the stresses than slow-growing cells. For example, slow-growing chemostat-cultivated cells (D = 0.08 h-1) and stationary-phase bacteria from batch culture that were exposed to mild heat had very similar T90 (time until 90% of the population is inactivated) values (T 90, chemostat = 2.66 h; T90, batch = 2.62 h), whereas T90 for cells growing at a μ of 0.9 h-1 was 0.2 h. We present evidence that the stress sensitivity of E. coli is correlated with the intracellular level of the alternative sigma factor RpoS. This is also supported by the fact that E. coli rpoS mutant cells were more stress sensitive than the parent strain by factors of 4.9 (mild heat), 5.3 (UVA light), and 4.1 (sunlight). Furthermore, modeling of inactivation curves with GInaFiT revealed that the shape of inactivation curves changed depending on the specific growth rate. Inactivation curves of cells from fast-growing cultures (μ = 1.0 h -1) that were irradiated with UVA light showed a tailing effect, while for slow-growing cultures (μ = 0.3 h-1), inactivation curves with shoulders were obtained. Our findings emphasize the need for accurate reporting of specific growth rates and detailed culture conditions in disinfection studies to allow comparison of data from different studies and laboratories and sound interpretation of the data obtained.

Original languageEnglish (US)
Pages (from-to)2586-2593
Number of pages8
JournalApplied and Environmental Microbiology
Volume72
Issue number4
DOIs
StatePublished - Apr 2006
Externally publishedYes

Fingerprint

Disinfection
disinfection
specific growth rate
Hot Temperature
Escherichia coli
heat
chemostat
Growth
inactivation
Sunlight
cells
solar radiation
Light
Sigma Factor
Batch Cell Culture Techniques
sigma factors
Drinking Water
tailings
shoulders
Cell Culture Techniques

ASJC Scopus subject areas

  • Environmental Science(all)
  • Biotechnology
  • Microbiology

Cite this

Specific growth rate determines the sensitivity of Escherichia coli to thermal, UVA, and solar disinfection. / Berney, Michael; Weilenmann, Hans Ulrich; Ihssen, Julian; Bassin, Claudio; Egli, Thomas.

In: Applied and Environmental Microbiology, Vol. 72, No. 4, 04.2006, p. 2586-2593.

Research output: Contribution to journalArticle

Berney, Michael ; Weilenmann, Hans Ulrich ; Ihssen, Julian ; Bassin, Claudio ; Egli, Thomas. / Specific growth rate determines the sensitivity of Escherichia coli to thermal, UVA, and solar disinfection. In: Applied and Environmental Microbiology. 2006 ; Vol. 72, No. 4. pp. 2586-2593.
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