Specific expression of insulin-like growth factor-II in rhabdomyosarcoma tumor cells

Caterina P. Minniti, M. Tsokos, W. A. Newton, L. J. Helman

Research output: Contribution to journalArticle

54 Citations (Scopus)

Abstract

Insulin-like growth factor II (IGF-II) acts as autocrine growth and motility factor in human rhabdomyosarcoma cell lines, and Northern blot analysis of tumor biopsy specimens from both alveolar and embryonal rhabdomyosarcoma demonstrates high levels of IGF-II mRNA expression. To determine the frequency and site of expression of IGF-II in these tumors, the authors performed in situ hybridization. All tumor specimens examined expressed the gene for IGF-II, and this expression was localized to the tumor cells themselves and not to the surrounding stroma. These data suggest that the IGF-II autocrine loop may be operating not only in vitro but also in vivo.

Original languageEnglish (US)
Pages (from-to)198-203
Number of pages6
JournalAmerican Journal of Clinical Pathology
Volume101
Issue number2
StatePublished - 1994
Externally publishedYes

Fingerprint

Insulin-Like Growth Factor II
Rhabdomyosarcoma
Neoplasms
Alveolar Rhabdomyosarcoma
Embryonal Rhabdomyosarcoma
Glucose-6-Phosphate Isomerase
Northern Blotting
In Situ Hybridization
Intercellular Signaling Peptides and Proteins
Biopsy
Cell Line
Messenger RNA
Genes

Keywords

  • In situ
  • Insulin-like growth factors
  • Rhabdomyosarcoma

ASJC Scopus subject areas

  • Pathology and Forensic Medicine

Cite this

Specific expression of insulin-like growth factor-II in rhabdomyosarcoma tumor cells. / Minniti, Caterina P.; Tsokos, M.; Newton, W. A.; Helman, L. J.

In: American Journal of Clinical Pathology, Vol. 101, No. 2, 1994, p. 198-203.

Research output: Contribution to journalArticle

@article{a3ec56c9103c4aa0981efaa6b3bdaccf,
title = "Specific expression of insulin-like growth factor-II in rhabdomyosarcoma tumor cells",
abstract = "Insulin-like growth factor II (IGF-II) acts as autocrine growth and motility factor in human rhabdomyosarcoma cell lines, and Northern blot analysis of tumor biopsy specimens from both alveolar and embryonal rhabdomyosarcoma demonstrates high levels of IGF-II mRNA expression. To determine the frequency and site of expression of IGF-II in these tumors, the authors performed in situ hybridization. All tumor specimens examined expressed the gene for IGF-II, and this expression was localized to the tumor cells themselves and not to the surrounding stroma. These data suggest that the IGF-II autocrine loop may be operating not only in vitro but also in vivo.",
keywords = "In situ, Insulin-like growth factors, Rhabdomyosarcoma",
author = "Minniti, {Caterina P.} and M. Tsokos and Newton, {W. A.} and Helman, {L. J.}",
year = "1994",
language = "English (US)",
volume = "101",
pages = "198--203",
journal = "American Journal of Clinical Pathology",
issn = "0002-9173",
publisher = "American Society of Clinical Pathologists",
number = "2",

}

TY - JOUR

T1 - Specific expression of insulin-like growth factor-II in rhabdomyosarcoma tumor cells

AU - Minniti, Caterina P.

AU - Tsokos, M.

AU - Newton, W. A.

AU - Helman, L. J.

PY - 1994

Y1 - 1994

N2 - Insulin-like growth factor II (IGF-II) acts as autocrine growth and motility factor in human rhabdomyosarcoma cell lines, and Northern blot analysis of tumor biopsy specimens from both alveolar and embryonal rhabdomyosarcoma demonstrates high levels of IGF-II mRNA expression. To determine the frequency and site of expression of IGF-II in these tumors, the authors performed in situ hybridization. All tumor specimens examined expressed the gene for IGF-II, and this expression was localized to the tumor cells themselves and not to the surrounding stroma. These data suggest that the IGF-II autocrine loop may be operating not only in vitro but also in vivo.

AB - Insulin-like growth factor II (IGF-II) acts as autocrine growth and motility factor in human rhabdomyosarcoma cell lines, and Northern blot analysis of tumor biopsy specimens from both alveolar and embryonal rhabdomyosarcoma demonstrates high levels of IGF-II mRNA expression. To determine the frequency and site of expression of IGF-II in these tumors, the authors performed in situ hybridization. All tumor specimens examined expressed the gene for IGF-II, and this expression was localized to the tumor cells themselves and not to the surrounding stroma. These data suggest that the IGF-II autocrine loop may be operating not only in vitro but also in vivo.

KW - In situ

KW - Insulin-like growth factors

KW - Rhabdomyosarcoma

UR - http://www.scopus.com/inward/record.url?scp=0028115727&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=0028115727&partnerID=8YFLogxK

M3 - Article

VL - 101

SP - 198

EP - 203

JO - American Journal of Clinical Pathology

JF - American Journal of Clinical Pathology

SN - 0002-9173

IS - 2

ER -