Specific changes in the oligosaccharide moieties of VSV grown in different lectin-resistant CHO cells

Margaret A. Robertson, James R. Etchison, James S. Robertson, Donald F. Summers, Pamela Stanley

Research output: Contribution to journalArticle

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Abstract

The carbohydrate moieties of the G glycoprotein of vesicular stomatitis virus (VSV) grown in three distinct lectin-resistant (LecR) Chinese hamster ovary (CHO) cell lines have been compared by fine structural analysis of radiolabeled glycopeptides. The mutant WgaRIII, selected for resistance to wheat germ agglutinin (WGA), produces VSV containing G glycoprotein specifically lacking in sialic acid. The mutant PhaRI, selected for resistance to phytohemagglutinin (PHA) and previously shown to lack a particular glycoprotein N-acetyl-glucosaminyl-transferase activity, produces VSV containing G glycoprotein specifically lacking terminal N-acetylglucosamine-galactose-sialic acid sequences and possessing an increased number of mannose residues in the "core" region of its carbohydrate moieties. The mutant PhaRIConARII, a "double" mutant selected from PhaRI cells for resistance to concanavalin A (ConA), produces VSV containing G glycoprotein with a further alteration in the mannose residues of the "core" oligosaccharide region. We discuss the relevance of these findings to the mechanisms of glycoprotein biosynthesis in mammalian cells and to the biochemical bases of lectin resistance in CHO cells.

Original languageEnglish (US)
Pages (from-to)515-526
Number of pages12
JournalCell
Volume13
Issue number3
DOIs
StatePublished - 1978
Externally publishedYes

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Vesicular Stomatitis
Cricetulus
Oligosaccharides
Viruses
Lectins
Ovary
Glycoproteins
N-Acetylneuraminic Acid
Mannose
Cells
Carbohydrates
Wheat Germ Agglutinins
Acetylglucosamine
Glycopeptides
Biosynthesis
Phytohemagglutinins
Concanavalin A
Transferases
Galactose
Structural analysis

ASJC Scopus subject areas

  • Cell Biology
  • Molecular Biology

Cite this

Specific changes in the oligosaccharide moieties of VSV grown in different lectin-resistant CHO cells. / Robertson, Margaret A.; Etchison, James R.; Robertson, James S.; Summers, Donald F.; Stanley, Pamela.

In: Cell, Vol. 13, No. 3, 1978, p. 515-526.

Research output: Contribution to journalArticle

Robertson, Margaret A. ; Etchison, James R. ; Robertson, James S. ; Summers, Donald F. ; Stanley, Pamela. / Specific changes in the oligosaccharide moieties of VSV grown in different lectin-resistant CHO cells. In: Cell. 1978 ; Vol. 13, No. 3. pp. 515-526.
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