Specific binding of a 125I-secretoneurin analogue to a human monocytic cell line

Clemens Schneitler, Christian Kähler, Christian J. Wiedermann, Ruth Hogue-Angeletti, Reiner Fischer-Colbrie

Research output: Contribution to journalArticle

38 Citations (Scopus)

Abstract

Secretoneurin (SN) is a novel neuropeptide expressed in the central and peripheral nervous system as well as in various endocrine tissues. SN inhibits growth of aortic pulmonary and endothelial cells and is a potent chemoattractant for endothelial cells, skin fibroblasts and monocytes. We investigated here the presence of specific high affinity binding sites for SN on a target tissue SN was iodinated with the Bolton-Hunter (BH) reagent and purified by isocratic reversed phase chromatography. Specific binding sites for 125I-BHSN were identified on human Mono Mac 6 cells, a monocytic cell line. Scatchard analysis revealed a single class of binding sites with a K(d) value of 7.3 nM and a B(max), of 322 (fmol/mg protein). Competition studies demonstrated that the 15 C-terminal amino acids of SN could displace authentic SN, whereas shorter fragments were inactive. Other sensory neuropeptides like substance P, calcitonin gene-related peptide or galamin as well as the chemokine receptor ligand Rantes or the typical chemoattractant FMLP could not displace SN. Our studies demonstrate specific high affinity binding sites for SN on a monocytic cell line. Since SN exerts a potent chemotactic activity towards monocytes and increases cytosolic calcium in these cells, these binding sites might well represent a putative functional plasma membrane receptor for SN.

Original languageEnglish (US)
Pages (from-to)87-91
Number of pages5
JournalJournal of Neuroimmunology
Volume86
Issue number1
DOIs
StatePublished - Jun 1 1998

Fingerprint

Cell Line
Binding Sites
Chemotactic Factors
Neuropeptides
Monocytes
Endothelial Cells
secretoneurin
Calcitonin Gene-Related Peptide
Chemokine Receptors
Peripheral Nervous System
Reverse-Phase Chromatography
Substance P
Central Nervous System
Fibroblasts
Cell Membrane
Ligands
Calcium
Amino Acids
Lung
Skin

Keywords

  • Chromogranins
  • Monocytes fischer- colbrieυibk.ac.at
  • Receptor
  • Secretogranin II

ASJC Scopus subject areas

  • Immunology
  • Immunology and Allergy
  • Clinical Neurology
  • Neurology

Cite this

Schneitler, C., Kähler, C., Wiedermann, C. J., Hogue-Angeletti, R., & Fischer-Colbrie, R. (1998). Specific binding of a 125I-secretoneurin analogue to a human monocytic cell line. Journal of Neuroimmunology, 86(1), 87-91. https://doi.org/10.1016/S0165-5728(98)00012-5

Specific binding of a 125I-secretoneurin analogue to a human monocytic cell line. / Schneitler, Clemens; Kähler, Christian; Wiedermann, Christian J.; Hogue-Angeletti, Ruth; Fischer-Colbrie, Reiner.

In: Journal of Neuroimmunology, Vol. 86, No. 1, 01.06.1998, p. 87-91.

Research output: Contribution to journalArticle

Schneitler, C, Kähler, C, Wiedermann, CJ, Hogue-Angeletti, R & Fischer-Colbrie, R 1998, 'Specific binding of a 125I-secretoneurin analogue to a human monocytic cell line', Journal of Neuroimmunology, vol. 86, no. 1, pp. 87-91. https://doi.org/10.1016/S0165-5728(98)00012-5
Schneitler C, Kähler C, Wiedermann CJ, Hogue-Angeletti R, Fischer-Colbrie R. Specific binding of a 125I-secretoneurin analogue to a human monocytic cell line. Journal of Neuroimmunology. 1998 Jun 1;86(1):87-91. https://doi.org/10.1016/S0165-5728(98)00012-5
Schneitler, Clemens ; Kähler, Christian ; Wiedermann, Christian J. ; Hogue-Angeletti, Ruth ; Fischer-Colbrie, Reiner. / Specific binding of a 125I-secretoneurin analogue to a human monocytic cell line. In: Journal of Neuroimmunology. 1998 ; Vol. 86, No. 1. pp. 87-91.
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