TY - JOUR
T1 - Special Section on Non-Coding RNAs in Clinical Practice
T2 - From Biomarkers to Therapeutic Tools Exosomal miR-145 and miR-885 Regulate Thrombosis in COVID-19
AU - Gambardella, Jessica
AU - Kansakar, Urna
AU - Sardu, Celestino
AU - Messina, Vincenzo
AU - Jankauskas, Stanislovas S.
AU - Marfella, Raffaele
AU - Maggi, Paolo
AU - Wang, Xujun
AU - Mone, Pasquale
AU - Paolisso, Giuseppe
AU - Sorriento, Daniela
AU - Santulli, Gaetano
N1 - Funding Information:
The Santulli’s Laboratory is supported in part by National Institutes of Health (NIH) National Institute of Diabetes and Digestive and Kidney Diseases (NIDDK) [Grant R01-DK123259] and [Grant R01-DK033823] and National Heart, Lung, and Blood Institute (NHLBI) [Grant R01-HL159062], [Grant R01-HL146691], and [Grant T32-HL144456] (to G.S.); the Diabetes Action Research and Education Foundation (to G.S.); and the Monique Weill-Caulier and Irma T. Hirschl Trusts (to G.S.). J.G. and S.S.J. are supported by a postdoctoral fellowship of the American Heart Association [AHA-20POST35211151] and [AHA-21POST836407], respectively.
Publisher Copyright:
© 2022 by The American Society for Pharmacology and Experimental Therapeutics.
PY - 2023/1/1
Y1 - 2023/1/1
N2 - We hypothesized that exosomal microRNAs could be implied in the pathogenesis of thromboembolic complications in coronavirus disease 2019 (COVID-19). We isolated circulating exosomes from patients with COVID-19, and then we divided our population in two arms based on the D-dimer level on hospital admission. We observed that exosomal miR-145 and miR-885 significantly correlate with D-dimer levels. Moreover, we demonstrate that human endothelial cells express the main cofactors needed for the internalization of the "Severe acute respiratory syndrome coronavirus 2"(SARS-CoV-2), including angiotensin converting enzyme 2, transmembrane protease serine 2, and CD-147. Interestingly, human endothelial cells treated with serum from COVID-19 patients release significantly less miR-145 and miR-885, exhibit increased apoptosis, and display significantly impaired angiogenetic properties compared with cells treated with non-COVID-19 serum. Taken together, our data indicate that exosomal miR-145 and miR-885 are essential in modulating thromboembolic events in COVID-19.
AB - We hypothesized that exosomal microRNAs could be implied in the pathogenesis of thromboembolic complications in coronavirus disease 2019 (COVID-19). We isolated circulating exosomes from patients with COVID-19, and then we divided our population in two arms based on the D-dimer level on hospital admission. We observed that exosomal miR-145 and miR-885 significantly correlate with D-dimer levels. Moreover, we demonstrate that human endothelial cells express the main cofactors needed for the internalization of the "Severe acute respiratory syndrome coronavirus 2"(SARS-CoV-2), including angiotensin converting enzyme 2, transmembrane protease serine 2, and CD-147. Interestingly, human endothelial cells treated with serum from COVID-19 patients release significantly less miR-145 and miR-885, exhibit increased apoptosis, and display significantly impaired angiogenetic properties compared with cells treated with non-COVID-19 serum. Taken together, our data indicate that exosomal miR-145 and miR-885 are essential in modulating thromboembolic events in COVID-19.
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U2 - 10.1124/jpet.122.001209
DO - 10.1124/jpet.122.001209
M3 - Article
C2 - 35772782
AN - SCOPUS:85136584429
SN - 0022-3565
VL - 384
SP - 109
EP - 115
JO - Journal of Pharmacology and Experimental Therapeutics
JF - Journal of Pharmacology and Experimental Therapeutics
IS - 1
ER -
