Spatial consequences of defective processing of specific yeast mRNAs revealed by fluorescent in situ hybridization

R. M. Long, D. U. Elliott, F. Stutz, M. Rosbash, Robert H. Singer

Research output: Contribution to journalArticle

72 Citations (Scopus)

Abstract

This work introduces the first use of fluorescent in situ hybridization (FISH) to detect the distribution of specific transcripts in Saccharomyces cerevisiae. We have applied this technique to analysis of reporter transcripts from a single, integrated copy, or multicopy plasmids. We have evaluated the effect of splice site deletions or the presence or absence of a terminator/cleavage site and demonstrated that both splicing and polyadenylation affect the export of these transcripts from the nucleus to the cytoplasm. Moreover, we show that the exported pre-mRNAs are substrates for nonsense codon-mediated decay through the UPF1 pathway. The work presented here demonstrates that the spatial distribution of transcripts will also be an important component of yeast RNA metabolism.

Original languageEnglish (US)
Pages (from-to)1071-1078
Number of pages8
JournalRNA
Volume1
Issue number10
StatePublished - 1995
Externally publishedYes

Fingerprint

Polyadenylation
Nonsense Codon
RNA Precursors
Fluorescence In Situ Hybridization
Saccharomyces cerevisiae
Cytoplasm
Plasmids
Yeasts
RNA
Messenger RNA

Keywords

  • Cleavage/polyadenylation
  • Nonsense codon-mediated decay
  • Nuclear transport
  • S. Cerevisiae

ASJC Scopus subject areas

  • Genetics
  • Molecular Biology

Cite this

Spatial consequences of defective processing of specific yeast mRNAs revealed by fluorescent in situ hybridization. / Long, R. M.; Elliott, D. U.; Stutz, F.; Rosbash, M.; Singer, Robert H.

In: RNA, Vol. 1, No. 10, 1995, p. 1071-1078.

Research output: Contribution to journalArticle

Long, R. M. ; Elliott, D. U. ; Stutz, F. ; Rosbash, M. ; Singer, Robert H. / Spatial consequences of defective processing of specific yeast mRNAs revealed by fluorescent in situ hybridization. In: RNA. 1995 ; Vol. 1, No. 10. pp. 1071-1078.
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