Sources of variability in mutant frequency determinations in different organs of lacZ plasmid-based transgenic mice: Experimental features and statistical analysis

Michaël E.T.I. Boerrigter, Jan Vijg

Research output: Contribution to journalArticle

14 Scopus citations


Sources of variability that may affect the measurement of mutant frequencies in lacZ plasmid-based transgenic mice, including rescue-to-rescue, animal-to-animal (within organ), and organ-to-organ, were studied in the context of some of the experimental features that distinguish this model from other currently used systems. Statistical analysis of repeated determinations of DNA from kidney, spleen, liver, lung, and brain indicated that 350,000 colony-forming units from each of three animals should be analyzed per treatment group in order to detect a 50% difference in mutant frequencies. Consideration is given to some of the experimental features of the transgenic assay system, including its positive selection system, its rescue efficiency, the ability to detect large deletions in the lacZ target genes, the contribution of Escherichia coil-derived mutations to the spontaneous mutant Frequencies observed in vivo, and cost effectiveness of mutant frequency determinations.

Original languageEnglish (US)
Pages (from-to)221-229
Number of pages9
JournalEnvironmental and Molecular Mutagenesis
Issue number3
StatePublished - May 29 1997
Externally publishedYes



  • Mutagenicity
  • Organ specificity
  • Plasmid rescue
  • Transgenic mouse
  • Variability

ASJC Scopus subject areas

  • Epidemiology
  • Genetics(clinical)
  • Health, Toxicology and Mutagenesis

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