Some electrical and pharmacological properties of gap junctions between adult ventricular myocytes.

R. L. White, David C. Spray, A. C. Campos de Carvalho, B. A. Wittenberg, M. V. Bennett

Research output: Contribution to journalArticle

84 Citations (Scopus)

Abstract

Ventricular myocytes were isolated from adult rat hearts using the technique of Wittenberg and Robinson (Cell Tissue Res. 216: 231-251, 1981). These cells exhibited morphology, input resistance, time constant, and excitability expected for cells in intact cardiac tissue. Pairs of these cells were electronically coupled, and junctional conductance was unaffected by transjunctional potential or hyperpolarization of both cells. Brief exposure of cell pairs to medium equilibrated with 100% CO2 or containing 0.1 mM octanol quickly and reversibly decreased junctional conductance. We conclude that gap junctions between pairs of ventricular myocytes possess physiological properties like those of junctions in many other tissues. This preparation will be useful in evaluating drug action on junctional communication in heart.

Original languageEnglish (US)
JournalThe American journal of physiology
Volume249
Issue number5 Pt 1
StatePublished - Nov 1985

Fingerprint

Gap Junctions
Muscle Cells
Pharmacology
Octanols
Communication
Pharmaceutical Preparations

ASJC Scopus subject areas

  • Medicine(all)

Cite this

White, R. L., Spray, D. C., Campos de Carvalho, A. C., Wittenberg, B. A., & Bennett, M. V. (1985). Some electrical and pharmacological properties of gap junctions between adult ventricular myocytes. The American journal of physiology, 249(5 Pt 1).

Some electrical and pharmacological properties of gap junctions between adult ventricular myocytes. / White, R. L.; Spray, David C.; Campos de Carvalho, A. C.; Wittenberg, B. A.; Bennett, M. V.

In: The American journal of physiology, Vol. 249, No. 5 Pt 1, 11.1985.

Research output: Contribution to journalArticle

White, RL, Spray, DC, Campos de Carvalho, AC, Wittenberg, BA & Bennett, MV 1985, 'Some electrical and pharmacological properties of gap junctions between adult ventricular myocytes.', The American journal of physiology, vol. 249, no. 5 Pt 1.
White, R. L. ; Spray, David C. ; Campos de Carvalho, A. C. ; Wittenberg, B. A. ; Bennett, M. V. / Some electrical and pharmacological properties of gap junctions between adult ventricular myocytes. In: The American journal of physiology. 1985 ; Vol. 249, No. 5 Pt 1.
@article{5ff0178d713841b5ac06ca88862d7ce6,
title = "Some electrical and pharmacological properties of gap junctions between adult ventricular myocytes.",
abstract = "Ventricular myocytes were isolated from adult rat hearts using the technique of Wittenberg and Robinson (Cell Tissue Res. 216: 231-251, 1981). These cells exhibited morphology, input resistance, time constant, and excitability expected for cells in intact cardiac tissue. Pairs of these cells were electronically coupled, and junctional conductance was unaffected by transjunctional potential or hyperpolarization of both cells. Brief exposure of cell pairs to medium equilibrated with 100{\%} CO2 or containing 0.1 mM octanol quickly and reversibly decreased junctional conductance. We conclude that gap junctions between pairs of ventricular myocytes possess physiological properties like those of junctions in many other tissues. This preparation will be useful in evaluating drug action on junctional communication in heart.",
author = "White, {R. L.} and Spray, {David C.} and {Campos de Carvalho}, {A. C.} and Wittenberg, {B. A.} and Bennett, {M. V.}",
year = "1985",
month = "11",
language = "English (US)",
volume = "249",
journal = "American Journal of Physiology - Renal Fluid and Electrolyte Physiology",
issn = "1931-857X",
publisher = "American Physiological Society",
number = "5 Pt 1",

}

TY - JOUR

T1 - Some electrical and pharmacological properties of gap junctions between adult ventricular myocytes.

AU - White, R. L.

AU - Spray, David C.

AU - Campos de Carvalho, A. C.

AU - Wittenberg, B. A.

AU - Bennett, M. V.

PY - 1985/11

Y1 - 1985/11

N2 - Ventricular myocytes were isolated from adult rat hearts using the technique of Wittenberg and Robinson (Cell Tissue Res. 216: 231-251, 1981). These cells exhibited morphology, input resistance, time constant, and excitability expected for cells in intact cardiac tissue. Pairs of these cells were electronically coupled, and junctional conductance was unaffected by transjunctional potential or hyperpolarization of both cells. Brief exposure of cell pairs to medium equilibrated with 100% CO2 or containing 0.1 mM octanol quickly and reversibly decreased junctional conductance. We conclude that gap junctions between pairs of ventricular myocytes possess physiological properties like those of junctions in many other tissues. This preparation will be useful in evaluating drug action on junctional communication in heart.

AB - Ventricular myocytes were isolated from adult rat hearts using the technique of Wittenberg and Robinson (Cell Tissue Res. 216: 231-251, 1981). These cells exhibited morphology, input resistance, time constant, and excitability expected for cells in intact cardiac tissue. Pairs of these cells were electronically coupled, and junctional conductance was unaffected by transjunctional potential or hyperpolarization of both cells. Brief exposure of cell pairs to medium equilibrated with 100% CO2 or containing 0.1 mM octanol quickly and reversibly decreased junctional conductance. We conclude that gap junctions between pairs of ventricular myocytes possess physiological properties like those of junctions in many other tissues. This preparation will be useful in evaluating drug action on junctional communication in heart.

UR - http://www.scopus.com/inward/record.url?scp=0022148962&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=0022148962&partnerID=8YFLogxK

M3 - Article

VL - 249

JO - American Journal of Physiology - Renal Fluid and Electrolyte Physiology

JF - American Journal of Physiology - Renal Fluid and Electrolyte Physiology

SN - 1931-857X

IS - 5 Pt 1

ER -