Somatically generated mouse myeloma variants synthesizing IgA half-molecules

D. J. Zack, S. L. Morrison, W. D. Cook, W. Dackowski, Matthew D. Scharff

Research output: Contribution to journalArticle

11 Citations (Scopus)

Abstract

Whereas mouse myelomas that secrete IgA half-molecules have been shown to arise in vivo, their origin has not been definitely established. We show that somatic variants secreting phenotypically similar molecules can arise directly from the normal IgA-secreting myelomas S107 and W3082. In addition to being improperly assembled, the variant proteins have distinct carboxy-terminal deletions and an aberrant heavy-light chain disulfide bond. For at least one of the variants, variable region serology and affinity for hapten are both unaffected by these changes. Southern and Northern blot analyses indicate normal size DNA restriction fragments and mRNA, suggesting premature termination as the mechanism of deletion. These results are discussed in relation to possible mutational hot spots and long-range interdomain interactions.

Original languageEnglish (US)
Pages (from-to)1554-1569
Number of pages16
JournalJournal of Experimental Medicine
Volume154
Issue number5
StatePublished - 1981
Externally publishedYes

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Immunoglobulin A
Haptens
Serology
Southern Blotting
Disulfides
Northern Blotting
Messenger RNA
DNA
Proteins

ASJC Scopus subject areas

  • Immunology

Cite this

Zack, D. J., Morrison, S. L., Cook, W. D., Dackowski, W., & Scharff, M. D. (1981). Somatically generated mouse myeloma variants synthesizing IgA half-molecules. Journal of Experimental Medicine, 154(5), 1554-1569.

Somatically generated mouse myeloma variants synthesizing IgA half-molecules. / Zack, D. J.; Morrison, S. L.; Cook, W. D.; Dackowski, W.; Scharff, Matthew D.

In: Journal of Experimental Medicine, Vol. 154, No. 5, 1981, p. 1554-1569.

Research output: Contribution to journalArticle

Zack, DJ, Morrison, SL, Cook, WD, Dackowski, W & Scharff, MD 1981, 'Somatically generated mouse myeloma variants synthesizing IgA half-molecules', Journal of Experimental Medicine, vol. 154, no. 5, pp. 1554-1569.
Zack, D. J. ; Morrison, S. L. ; Cook, W. D. ; Dackowski, W. ; Scharff, Matthew D. / Somatically generated mouse myeloma variants synthesizing IgA half-molecules. In: Journal of Experimental Medicine. 1981 ; Vol. 154, No. 5. pp. 1554-1569.
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