Somatic mutation of the T15 heavy chain gives rise to an antibody with autoantibody specificity

B. Diamond, Matthew D. Scharff

Research output: Contribution to journalArticle

263 Citations (Scopus)

Abstract

The S107 IgA κ-chain myeloma cell line makes an antiphosphocholine antibody of the T15 idiotype. A somatic mutant of this line, U4, makes an immunoglobulin with a single amino acid substitution of an alanine for a glutamic acid at residue 35. This single amino acid change results in a loss of phosphocholine binding activity. However, the U4 immunoglobulin has acquired reactivity with a variety of phosphorylated macromolecules, including double-stranded DNA, protamine, and cardiolipin. Thus, a single amino acid change in the T15 heavy chain can transform an antibacterial antibody into an antibody that resembles the autoantibodies seen in mice and man with autoimmune disease.

Original languageEnglish (US)
Pages (from-to)5841-5844
Number of pages4
JournalProceedings of the National Academy of Sciences of the United States of America
Volume81
Issue number18 I
StatePublished - 1984

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Autoantibodies
Mutation
Antibodies
Immunoglobulins
Amino Acids
Cardiolipins
Protamines
Phosphorylcholine
Amino Acid Substitution
Alanine
Immunoglobulin A
Autoimmune Diseases
Glutamic Acid
Cell Line
DNA

ASJC Scopus subject areas

  • General
  • Genetics

Cite this

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AB - The S107 IgA κ-chain myeloma cell line makes an antiphosphocholine antibody of the T15 idiotype. A somatic mutant of this line, U4, makes an immunoglobulin with a single amino acid substitution of an alanine for a glutamic acid at residue 35. This single amino acid change results in a loss of phosphocholine binding activity. However, the U4 immunoglobulin has acquired reactivity with a variety of phosphorylated macromolecules, including double-stranded DNA, protamine, and cardiolipin. Thus, a single amino acid change in the T15 heavy chain can transform an antibacterial antibody into an antibody that resembles the autoantibodies seen in mice and man with autoimmune disease.

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