Somatic diversification of the S107 (T15) V(H)11 germ-line gene that encodes the heavy-chain variable region of antibodies to double-stranded DNA in (NZB x NZW)F1 mice

S. M. Behar; M. D. Scharff

doi:10.1073/pnas.85.11.3970

Somatic diversification of the S107 (T15) V(H)11 germ-line gene that encodes the heavy-chain variable region of antibodies to double-stranded DNA in (NZB x NZW)F₁ mice

S. M. Behar, M. D. Scharff

Cell Biology

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Abstract

A variety of studies suggest that members of the S107 (T15) heavy-chain variable-region gene family contribute to the autoimmune response of mice and humans to DNA. To identify the germ-line gene(s) involved and the degree of somatic diversification that occurs in such autoantibodies, we determined the mRNA sequence of the heavy and light chains of a group of monoclonal anti-DNA antibodies encoded by the S107 V(H)11 germ-line gene in (NZB x NZW)F₁ mice. We also cloned and sequenced the V(H)11 germ-line gene of the NZB and NZW parental strains. The V(H)11 coding sequences of the two strains were identical. Comparison with this heavy-chain germ-line sequence showed that the variable regions of the monoclonal antibodies had undergone considerable somatic diversification.

Original language	English (US)
Pages (from-to)	3970-3974
Number of pages	5
Journal	Proceedings of the National Academy of Sciences of the United States of America
Volume	85
Issue number	11
DOIs	https://doi.org/10.1073/pnas.85.11.3970
State	Published - 1988

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Somatic diversification of the S107 (T15) V(H)11 germ-line gene that encodes the heavy-chain variable region of antibodies to double-stranded DNA in (NZB x NZW)F₁ mice. / Behar, S. M.; Scharff, M. D.
In: Proceedings of the National Academy of Sciences of the United States of America, Vol. 85, No. 11, 1988, p. 3970-3974.

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Somatic diversification of the S107 (T15) V(H)11 germ-line gene that encodes the heavy-chain variable region of antibodies to double-stranded DNA in (NZB x NZW)F₁ mice

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