Somatic diversification of the S107 (T15) V(H)11 germ-line gene that encodes the heavy-chain variable region of antibodies to double-stranded DNA in (NZB x NZW)F1 mice

S. M. Behar, Matthew D. Scharff

Research output: Contribution to journalArticle

40 Citations (Scopus)

Abstract

A variety of studies suggest that members of the S107 (T15) heavy-chain variable-region gene family contribute to the autoimmune response of mice and humans to DNA. To identify the germ-line gene(s) involved and the degree of somatic diversification that occurs in such autoantibodies, we determined the mRNA sequence of the heavy and light chains of a group of monoclonal anti-DNA antibodies encoded by the S107 V(H)11 germ-line gene in (NZB x NZW)F1 mice. We also cloned and sequenced the V(H)11 germ-line gene of the NZB and NZW parental strains. The V(H)11 coding sequences of the two strains were identical. Comparison with this heavy-chain germ-line sequence showed that the variable regions of the monoclonal antibodies had undergone considerable somatic diversification.

Original languageEnglish (US)
Pages (from-to)3970-3974
Number of pages5
JournalProceedings of the National Academy of Sciences of the United States of America
Volume85
Issue number11
StatePublished - 1988

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Germ Cells
Antibodies
DNA
Genes
Monoclonal Antibodies
Antinuclear Antibodies
Autoimmunity
Autoantibodies
Light
Messenger RNA

ASJC Scopus subject areas

  • General
  • Genetics

Cite this

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AB - A variety of studies suggest that members of the S107 (T15) heavy-chain variable-region gene family contribute to the autoimmune response of mice and humans to DNA. To identify the germ-line gene(s) involved and the degree of somatic diversification that occurs in such autoantibodies, we determined the mRNA sequence of the heavy and light chains of a group of monoclonal anti-DNA antibodies encoded by the S107 V(H)11 germ-line gene in (NZB x NZW)F1 mice. We also cloned and sequenced the V(H)11 germ-line gene of the NZB and NZW parental strains. The V(H)11 coding sequences of the two strains were identical. Comparison with this heavy-chain germ-line sequence showed that the variable regions of the monoclonal antibodies had undergone considerable somatic diversification.

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