Somatic diversification of the S107 (T15) V(H)11 germ-line gene that encodes the heavy-chain variable region of antibodies to double-stranded DNA in (NZB x NZW)F1 mice

S. M. Behar, M. D. Scharff

Research output: Contribution to journalArticle

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Abstract

A variety of studies suggest that members of the S107 (T15) heavy-chain variable-region gene family contribute to the autoimmune response of mice and humans to DNA. To identify the germ-line gene(s) involved and the degree of somatic diversification that occurs in such autoantibodies, we determined the mRNA sequence of the heavy and light chains of a group of monoclonal anti-DNA antibodies encoded by the S107 V(H)11 germ-line gene in (NZB x NZW)F1 mice. We also cloned and sequenced the V(H)11 germ-line gene of the NZB and NZW parental strains. The V(H)11 coding sequences of the two strains were identical. Comparison with this heavy-chain germ-line sequence showed that the variable regions of the monoclonal antibodies had undergone considerable somatic diversification.

Original languageEnglish (US)
Pages (from-to)3970-3974
Number of pages5
JournalProceedings of the National Academy of Sciences of the United States of America
Volume85
Issue number11
DOIs
StatePublished - 1988

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