TY - JOUR
T1 - Somatic Cell Genetic Analysis of Myelomas and Hybridomas
AU - French, Deborah
AU - Kelly, Theresa
AU - Buhl, Susan
AU - Scharff, Matthew D.
PY - 1987/1
Y1 - 1987/1
N2 - The study and genetic manipulation of hybridoma cells growing in culture have acquired great practical significance. As with parental myeloma cells, mouse, rat, and human hybridomas grow as suspended cells. The best hybridomas produce about 50 μg of antibody/106 cells/24 hr and usually have doubling times of 17–19 hr. They are commonly grown in petri or tissue culture dishes in CO2 incubators but may also be propagated in large amounts in any of a variety of culture vessels. A few mouse myelomas were originally made with derivatives of MPC 11 but these have similar characteristics. There are still considerable problems with making human monoclonal antibodies and an ideal fusion parent has not yet been found. The chapter discusses mouse and rat myelomas having similar characteristics.
AB - The study and genetic manipulation of hybridoma cells growing in culture have acquired great practical significance. As with parental myeloma cells, mouse, rat, and human hybridomas grow as suspended cells. The best hybridomas produce about 50 μg of antibody/106 cells/24 hr and usually have doubling times of 17–19 hr. They are commonly grown in petri or tissue culture dishes in CO2 incubators but may also be propagated in large amounts in any of a variety of culture vessels. A few mouse myelomas were originally made with derivatives of MPC 11 but these have similar characteristics. There are still considerable problems with making human monoclonal antibodies and an ideal fusion parent has not yet been found. The chapter discusses mouse and rat myelomas having similar characteristics.
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U2 - 10.1016/S0076-6879(87)51008-4
DO - 10.1016/S0076-6879(87)51008-4
M3 - Article
C2 - 3431452
AN - SCOPUS:0023623048
SN - 0076-6879
VL - 151
SP - 50
EP - 66
JO - Methods in Enzymology
JF - Methods in Enzymology
IS - C
ER -