Somatic Cell Genetic Analysis of Myelomas and Hybridomas

Deborah French, Theresa Kelly, Susan Buhl, Matthew D. Scharff

Research output: Contribution to journalArticle

11 Scopus citations

Abstract

The study and genetic manipulation of hybridoma cells growing in culture have acquired great practical significance. As with parental myeloma cells, mouse, rat, and human hybridomas grow as suspended cells. The best hybridomas produce about 50 μg of antibody/106 cells/24 hr and usually have doubling times of 17–19 hr. They are commonly grown in petri or tissue culture dishes in CO2 incubators but may also be propagated in large amounts in any of a variety of culture vessels. A few mouse myelomas were originally made with derivatives of MPC 11 but these have similar characteristics. There are still considerable problems with making human monoclonal antibodies and an ideal fusion parent has not yet been found. The chapter discusses mouse and rat myelomas having similar characteristics.

Original languageEnglish (US)
Pages (from-to)50-66
Number of pages17
JournalMethods in Enzymology
Volume151
Issue numberC
DOIs
StatePublished - Jan 1987

ASJC Scopus subject areas

  • Biochemistry
  • Molecular Biology

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