Solution structure of the proapoptotic molecule BID

A structural basis for apoptotic agonists and antagonists

James M. McDonnell, David Fushman, Curt L. Milliman, Stanley J. Korsmeyer, David Cowburn

Research output: Contribution to journalArticle

319 Citations (Scopus)

Abstract

Members of the BCL2 family of proteins are key regulators of programmed cell death, acting either as apoptotic agonists or antagonists. Here we describe the solution structure of BID, presenting the structure of a proapoptotic BCL2 family member. An analysis of sequence/structure of BCL2 family members allows us to define a structural superfamily, which has implications for general mechanisms for regulating proapoptotic activity. It appears two criteria must be met for proapoptotic function within the BCL2 family: targeting of molecules to intracellular membranes, and exposure of the BH3 death domain. BID's activity is regulated by a Caspase 8-mediated cleavage event, exposing the BH3 domain and significantly changing the surface charge and hydrophobicity, resulting in a change of cellular localization.

Original languageEnglish (US)
Pages (from-to)625-634
Number of pages10
JournalCell
Volume96
Issue number5
StatePublished - Mar 5 1999
Externally publishedYes

Fingerprint

Proto-Oncogene Proteins c-bcl-2
Caspase 8
Cell death
Surface charge
Hydrophobicity
Membranes
Molecules
Intracellular Membranes
Hydrophobic and Hydrophilic Interactions
Sequence Analysis
Cell Death

ASJC Scopus subject areas

  • Cell Biology
  • Molecular Biology

Cite this

McDonnell, J. M., Fushman, D., Milliman, C. L., Korsmeyer, S. J., & Cowburn, D. (1999). Solution structure of the proapoptotic molecule BID: A structural basis for apoptotic agonists and antagonists. Cell, 96(5), 625-634.

Solution structure of the proapoptotic molecule BID : A structural basis for apoptotic agonists and antagonists. / McDonnell, James M.; Fushman, David; Milliman, Curt L.; Korsmeyer, Stanley J.; Cowburn, David.

In: Cell, Vol. 96, No. 5, 05.03.1999, p. 625-634.

Research output: Contribution to journalArticle

McDonnell, JM, Fushman, D, Milliman, CL, Korsmeyer, SJ & Cowburn, D 1999, 'Solution structure of the proapoptotic molecule BID: A structural basis for apoptotic agonists and antagonists', Cell, vol. 96, no. 5, pp. 625-634.
McDonnell, James M. ; Fushman, David ; Milliman, Curt L. ; Korsmeyer, Stanley J. ; Cowburn, David. / Solution structure of the proapoptotic molecule BID : A structural basis for apoptotic agonists and antagonists. In: Cell. 1999 ; Vol. 96, No. 5. pp. 625-634.
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