Sodium channels from human brain RNA expressed in Xenopus oocyte. Basic electrophysiologic characteristics and their modification by diphenylhydantoin

G. F. Tomaselli, E. Marban, G. Yellen

Research output: Contribution to journalArticle

30 Scopus citations

Abstract

We described the expression and characterization of sodium channels from human brain RNA in the Xenopus oocyte. The expressed channel, studied by whole-cell voltage clamp, reveals characteristic selectivity for sodium as the permeant ion, voltage-dependent gating, and block by nanomolar concentrations of tetrodotoxin. Such channels are not seen in control oocytes injected with solvent only. The anticonvulsant diphenylhydantoin (DPH) inhibits the expressed channel in a voltage- and use-dependent manner, much like the effect seen in primary mammalian neuronal preparations. The inhibition of the expressed human sodium channel by DPH can be described by models previously developed to explain block of Na channels by local anesthetics. The preferential block of Na channels during depolarization helps explain the selectivity of DPH for neurons involved in seizure activity.

Original languageEnglish (US)
Pages (from-to)1724-1732
Number of pages9
JournalJournal of Clinical Investigation
Volume83
Issue number5
DOIs
StatePublished - 1989

ASJC Scopus subject areas

  • Medicine(all)

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