SNARE-mediated fusion of liposomes

Jérôme Vicogne, Jeffrey E. Pessin

Research output: Chapter in Book/Report/Conference proceedingChapter

2 Scopus citations

Abstract

Lipid-mixing assay is now commonly used to study protein, temperature and ion-dependent membrane fusion events. This assay has been crucial to demonstrate the ability of neuronal and non-neuronal soluble NSF attachment receptor (SNARE) to promote spontaneous fusion of liposomes. This lipid-mixing assay is based on the fluorescence resonance energy transfer (FRET) capability between a donor fluorescent lipid and a quenching lipid. When fusion between donor fluorescent liposomes and nonfluorescent acceptor liposome occurred, FRET decreases. This assay allows a real-time reading of SNARE-mediated liposome fusion.

Original languageEnglish (US)
Title of host publicationMembrane Trafficking
PublisherHumana Press Inc.
Pages241-251
Number of pages11
ISBN (Print)9781588299253
DOIs
StatePublished - Jan 1 2008
Externally publishedYes

Publication series

NameMethods in Molecular Biology
Volume457
ISSN (Print)1064-3745

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Keywords

  • FRET
  • Fusion
  • Lipids
  • Liposomes
  • SNARE

ASJC Scopus subject areas

  • Molecular Biology
  • Genetics

Cite this

Vicogne, J., & Pessin, J. E. (2008). SNARE-mediated fusion of liposomes. In Membrane Trafficking (pp. 241-251). (Methods in Molecular Biology; Vol. 457). Humana Press Inc.. https://doi.org/10.1007/978-1-59745-261-8_18