SNARE-mediated fusion of liposomes.

Jérôme Vicogne, Jeffrey E. Pessin

Research output: Contribution to journalArticle

Abstract

Lipid-mixing assay is now commonly used to study protein, temperature and ion-dependent membrane fusion events. This assay has been crucial to demonstrate the ability of neuronal and non-neuronal soluble NSF attachment receptor (SNARE) to promote spontaneous fusion of liposomes. This lipid-mixing assay is based on the fluorescence resonance energy transfer (FRET) capability between a donor fluorescent lipid and a quenching lipid. When fusion between donor fluorescent liposomes and nonfluorescent acceptor liposome occurred, FRET decreases. This assay allows a real-time reading of SNARE-mediated liposome fusion.

Original languageEnglish (US)
Pages (from-to)241-251
Number of pages11
JournalMethods in molecular biology (Clifton, N.J.)
Volume457
StatePublished - 2008
Externally publishedYes

ASJC Scopus subject areas

  • Molecular Biology
  • Genetics

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