Smooth muscle cell expression of a constitutive active form of human Rac 1 accelerates cutaneous wound repair

Hamdy H. Hassanain, Fawzi Irshaid, Sheik Wisel, John Sheridan, Robert E. Michler, Pascal J. Goldschmidt-Clermont

Research output: Contribution to journalArticle

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Abstract

Hyperoxia has been shown to improve wound healing; however, the mechanism for such therapeutic effects of oxygen remains hypothetical. Rac 1 regulates a wide variety of cellular activities, including cell proliferation and migration, and also is a key regulator for the activity of the nicotinamide dinucleotide phosphate oxidase the enzyme complex responsible for the production of a large fraction of cellular superoxide. We generated transgenic mice that express either the cDNA of a constitutively active mutant of human Rac 1 (V12 mutant or Rac CA) or the dominant negative isoform (V12 and N17 mutant or Rac DN) in the blood vessels using mouse vascular smooth muscle promoter for α-actin. We placed 2 wounds of 6 mm in diameter at the middorsal region of each mouse and allowed about 3 weeks for the wounds to heal. The size of the wounds in Rac CA transgenic mice was reduced relative to wild type mice; healing of Rac DN mice was slower than wild type and Rac CA (P <. 05). Blood vessel formation appeared faster in Rac CA mice, a finding associated with enhanced expression of some angiogenic growth factors. The current studies suggest that Rac 1 activation accelerates the wound healing process and is associated with more efficient angiogenesis at the wound site.

Original languageEnglish (US)
Pages (from-to)92-101
Number of pages10
JournalSurgery
Volume137
Issue number1
DOIs
StatePublished - Jan 2005
Externally publishedYes

Fingerprint

Smooth Muscle Myocytes
Skin
Wounds and Injuries
Wound Healing
Transgenic Mice
Blood Vessels
Hyperoxia
Niacinamide
Angiogenesis Inducing Agents
Therapeutic Uses
Vascular Smooth Muscle
Superoxides
Cell Movement
Actins
Intercellular Signaling Peptides and Proteins
Oxidoreductases
Protein Isoforms
Complementary DNA
Phosphates
Cell Proliferation

ASJC Scopus subject areas

  • Surgery

Cite this

Smooth muscle cell expression of a constitutive active form of human Rac 1 accelerates cutaneous wound repair. / Hassanain, Hamdy H.; Irshaid, Fawzi; Wisel, Sheik; Sheridan, John; Michler, Robert E.; Goldschmidt-Clermont, Pascal J.

In: Surgery, Vol. 137, No. 1, 01.2005, p. 92-101.

Research output: Contribution to journalArticle

Hassanain, HH, Irshaid, F, Wisel, S, Sheridan, J, Michler, RE & Goldschmidt-Clermont, PJ 2005, 'Smooth muscle cell expression of a constitutive active form of human Rac 1 accelerates cutaneous wound repair', Surgery, vol. 137, no. 1, pp. 92-101. https://doi.org/10.1016/j.surg.2004.06.012
Hassanain, Hamdy H. ; Irshaid, Fawzi ; Wisel, Sheik ; Sheridan, John ; Michler, Robert E. ; Goldschmidt-Clermont, Pascal J. / Smooth muscle cell expression of a constitutive active form of human Rac 1 accelerates cutaneous wound repair. In: Surgery. 2005 ; Vol. 137, No. 1. pp. 92-101.
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