Smooth muscle cell expression of a constitutive active form of human Rac 1 accelerates cutaneous wound repair

Hamdy H. Hassanain, Fawzi Irshaid, Sheik Wisel, John Sheridan, Robert E. Michler, Pascal J. Goldschmidt-Clermont

Research output: Contribution to journalArticle

14 Scopus citations

Abstract

Hyperoxia has been shown to improve wound healing; however, the mechanism for such therapeutic effects of oxygen remains hypothetical. Rac 1 regulates a wide variety of cellular activities, including cell proliferation and migration, and also is a key regulator for the activity of the nicotinamide dinucleotide phosphate oxidase the enzyme complex responsible for the production of a large fraction of cellular superoxide. We generated transgenic mice that express either the cDNA of a constitutively active mutant of human Rac 1 (V12 mutant or Rac CA) or the dominant negative isoform (V12 and N17 mutant or Rac DN) in the blood vessels using mouse vascular smooth muscle promoter for α-actin. We placed 2 wounds of 6 mm in diameter at the middorsal region of each mouse and allowed about 3 weeks for the wounds to heal. The size of the wounds in Rac CA transgenic mice was reduced relative to wild type mice; healing of Rac DN mice was slower than wild type and Rac CA (P <. 05). Blood vessel formation appeared faster in Rac CA mice, a finding associated with enhanced expression of some angiogenic growth factors. The current studies suggest that Rac 1 activation accelerates the wound healing process and is associated with more efficient angiogenesis at the wound site.

Original languageEnglish (US)
Pages (from-to)92-101
Number of pages10
JournalSurgery
Volume137
Issue number1
DOIs
Publication statusPublished - Jan 1 2005
Externally publishedYes

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ASJC Scopus subject areas

  • Surgery

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