Abstract
Hyperoxia has been shown to improve wound healing; however, the mechanism for such therapeutic effects of oxygen remains hypothetical. Rac 1 regulates a wide variety of cellular activities, including cell proliferation and migration, and also is a key regulator for the activity of the nicotinamide dinucleotide phosphate oxidase the enzyme complex responsible for the production of a large fraction of cellular superoxide. We generated transgenic mice that express either the cDNA of a constitutively active mutant of human Rac 1 (V12 mutant or Rac CA) or the dominant negative isoform (V12 and N17 mutant or Rac DN) in the blood vessels using mouse vascular smooth muscle promoter for α-actin. We placed 2 wounds of 6 mm in diameter at the middorsal region of each mouse and allowed about 3 weeks for the wounds to heal. The size of the wounds in Rac CA transgenic mice was reduced relative to wild type mice; healing of Rac DN mice was slower than wild type and Rac CA (P <. 05). Blood vessel formation appeared faster in Rac CA mice, a finding associated with enhanced expression of some angiogenic growth factors. The current studies suggest that Rac 1 activation accelerates the wound healing process and is associated with more efficient angiogenesis at the wound site.
| Original language | English (US) |
|---|---|
| Pages (from-to) | 92-101 |
| Number of pages | 10 |
| Journal | Surgery |
| Volume | 137 |
| Issue number | 1 |
| DOIs | |
| State | Published - Jan 2005 |
| Externally published | Yes |
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ASJC Scopus subject areas
- Surgery
Cite this
Smooth muscle cell expression of a constitutive active form of human Rac 1 accelerates cutaneous wound repair. / Hassanain, Hamdy H.; Irshaid, Fawzi; Wisel, Sheik; Sheridan, John; Michler, Robert E.; Goldschmidt-Clermont, Pascal J.
In: Surgery, Vol. 137, No. 1, 01.2005, p. 92-101.Research output: Contribution to journal › Article
}
TY - JOUR
T1 - Smooth muscle cell expression of a constitutive active form of human Rac 1 accelerates cutaneous wound repair
AU - Hassanain, Hamdy H.
AU - Irshaid, Fawzi
AU - Wisel, Sheik
AU - Sheridan, John
AU - Michler, Robert E.
AU - Goldschmidt-Clermont, Pascal J.
PY - 2005/1
Y1 - 2005/1
N2 - Hyperoxia has been shown to improve wound healing; however, the mechanism for such therapeutic effects of oxygen remains hypothetical. Rac 1 regulates a wide variety of cellular activities, including cell proliferation and migration, and also is a key regulator for the activity of the nicotinamide dinucleotide phosphate oxidase the enzyme complex responsible for the production of a large fraction of cellular superoxide. We generated transgenic mice that express either the cDNA of a constitutively active mutant of human Rac 1 (V12 mutant or Rac CA) or the dominant negative isoform (V12 and N17 mutant or Rac DN) in the blood vessels using mouse vascular smooth muscle promoter for α-actin. We placed 2 wounds of 6 mm in diameter at the middorsal region of each mouse and allowed about 3 weeks for the wounds to heal. The size of the wounds in Rac CA transgenic mice was reduced relative to wild type mice; healing of Rac DN mice was slower than wild type and Rac CA (P <. 05). Blood vessel formation appeared faster in Rac CA mice, a finding associated with enhanced expression of some angiogenic growth factors. The current studies suggest that Rac 1 activation accelerates the wound healing process and is associated with more efficient angiogenesis at the wound site.
AB - Hyperoxia has been shown to improve wound healing; however, the mechanism for such therapeutic effects of oxygen remains hypothetical. Rac 1 regulates a wide variety of cellular activities, including cell proliferation and migration, and also is a key regulator for the activity of the nicotinamide dinucleotide phosphate oxidase the enzyme complex responsible for the production of a large fraction of cellular superoxide. We generated transgenic mice that express either the cDNA of a constitutively active mutant of human Rac 1 (V12 mutant or Rac CA) or the dominant negative isoform (V12 and N17 mutant or Rac DN) in the blood vessels using mouse vascular smooth muscle promoter for α-actin. We placed 2 wounds of 6 mm in diameter at the middorsal region of each mouse and allowed about 3 weeks for the wounds to heal. The size of the wounds in Rac CA transgenic mice was reduced relative to wild type mice; healing of Rac DN mice was slower than wild type and Rac CA (P <. 05). Blood vessel formation appeared faster in Rac CA mice, a finding associated with enhanced expression of some angiogenic growth factors. The current studies suggest that Rac 1 activation accelerates the wound healing process and is associated with more efficient angiogenesis at the wound site.
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UR - http://www.scopus.com/inward/citedby.url?scp=11144335896&partnerID=8YFLogxK
U2 - 10.1016/j.surg.2004.06.012
DO - 10.1016/j.surg.2004.06.012
M3 - Article
VL - 137
SP - 92
EP - 101
JO - Surgery (United States)
JF - Surgery (United States)
SN - 0039-6060
IS - 1
ER -