Small GTP-binding protein TC10 differentially regulates two distinct populations of filamentous actin in 3T3L1 adipocytes

Makoto Kanzaki, Robert T. Watson, June Chunqiu Hou, Mark Stamnes, Alan R. Saltiel, Jeffrey E. Pessin

Research output: Contribution to journalArticle

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Abstract

TC10 is a member of the Rho family of small GTP-binding proteins that has previously been implicated in the regulation of insulin-stimulated GLUT4 translocation in adipocytes. In a manner similar to Cdc42-stimulated actin-based motility, we have observed that constitutively active TC10 (TC10/Q75L) can induce actin comet tails in Xenopus oocyte extracts in vitro and extensive actin polymerization in the perinuclear region when expressed in 3T3L1 adipocytes. In contrast, expression of TC10/Q75L completely disrupted adipocyte cortical actin, which was specific for TC10, because expression of constitutively active Cdc42 was without effect. The effect of TC10/Q75L to disrupt cortical actin was abrogated after deletion of the amino terminal extension (ΔN-TC10/Q75L), whereas this deletion retained the ability to induce perinuclear actin polymerization. In addition, alteration of perinuclear actin by expression of TC10/Q75L, a dominant-interfering TC10/T31N mutant or a mutant N-WASP protein (N-WASP/ΔVCA) reduced the rate of VSV G protein trafficking to the plasma membrane. Furthermore, TC10 directly bound to Golgi COPI coat proteins through a dilysine motif in the carboxyl terminal domain consistent with a role for TC10 regulating actin polymerization on membrane transport vesicles. Together, these data demonstrate that TC10 can differentially regulate two types of filamentous actin in adipocytes dependent on distinct functional domains and its subcellular compartmentalization.

Original languageEnglish (US)
Pages (from-to)2334-2346
Number of pages13
JournalMolecular Biology of the Cell
Volume13
Issue number7
DOIs
StatePublished - 2002
Externally publishedYes

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GTP-Binding Proteins
Adipocytes
Actins
Population
Polymerization
Neuronal Wiskott-Aldrich Syndrome Protein
lysyllysine
Coat Protein Complex I
Transport Vesicles
rho GTP-Binding Proteins
Aptitude
Capsid Proteins
Protein Transport
Mutant Proteins
Xenopus
Oocytes
Tail
Cell Membrane
Insulin
Membranes

ASJC Scopus subject areas

  • Cell Biology
  • Genetics
  • Molecular Biology

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Small GTP-binding protein TC10 differentially regulates two distinct populations of filamentous actin in 3T3L1 adipocytes. / Kanzaki, Makoto; Watson, Robert T.; Hou, June Chunqiu; Stamnes, Mark; Saltiel, Alan R.; Pessin, Jeffrey E.

In: Molecular Biology of the Cell, Vol. 13, No. 7, 2002, p. 2334-2346.

Research output: Contribution to journalArticle

Kanzaki, Makoto ; Watson, Robert T. ; Hou, June Chunqiu ; Stamnes, Mark ; Saltiel, Alan R. ; Pessin, Jeffrey E. / Small GTP-binding protein TC10 differentially regulates two distinct populations of filamentous actin in 3T3L1 adipocytes. In: Molecular Biology of the Cell. 2002 ; Vol. 13, No. 7. pp. 2334-2346.
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