SMAD3 represses androgen receptor-mediated transcription

S. A. Hayes, M. Zarnegar, M. Sharma, Fajun Yang, D. M. Peehl, P. Ten Dijke, Z. Sun

Research output: Contribution to journalArticle

162 Citations (Scopus)

Abstract

The androgen-signaling pathway is important in the growth and progression of prostate cancer. Androgen ablation therapy, which may result in programmed cell death, is often used to treat advanced prostate cancer. The growth-promoting effects of androgen are mediated mostly through the androgen receptor (AR). Transforming growth factor β (TGF-β) plays critical roles in controlling prostate cell proliferation, differentiation, and apoptosis. Normal transcripts and proteins of TGF-β receptors are frequently lost in prostate cancer cells, especially in advanced stages of the disease. However, the mechanisms by which TGF-β inhibits proliferation and induces apoptosis in prostate cancer cells is not clear. We investigated the molecular mechanism by which TGF-β inhibits transcriptional activation mediated by AR. Using transient transfection systems, we demonstrated that Smad3 specifically represses transcriptional activation mediated by AR on two natural androgen-responsive promoters. This repression is transmitted through TGF-β signaling and can be regulated by other Smad proteins. A protein-protein interaction between AR and Smad3 was identified in vitro and in vivo, and the transcription activation domain of AR and the MH2 of Smad3 were identified as being responsible for binding. Additional functional experiments showed that the repression of AR by Smad3 is mediated solely through the MH2 domain. These results provide fresh insight for understanding the mechanism by which TGF-β regulates the androgen-signaling pathway in prostate cancer cells.

Original languageEnglish (US)
Pages (from-to)2112-2118
Number of pages7
JournalCancer Research
Volume61
Issue number5
StatePublished - Mar 1 2001
Externally publishedYes

Fingerprint

Androgen Receptors
Transforming Growth Factors
Androgens
Prostatic Neoplasms
Transcriptional Activation
Smad Proteins
Apoptosis
Proteins
Growth Factor Receptors
Growth
Transfection
Prostate
Cell Differentiation
Cell Death
Cell Proliferation

ASJC Scopus subject areas

  • Cancer Research
  • Oncology

Cite this

Hayes, S. A., Zarnegar, M., Sharma, M., Yang, F., Peehl, D. M., Ten Dijke, P., & Sun, Z. (2001). SMAD3 represses androgen receptor-mediated transcription. Cancer Research, 61(5), 2112-2118.

SMAD3 represses androgen receptor-mediated transcription. / Hayes, S. A.; Zarnegar, M.; Sharma, M.; Yang, Fajun; Peehl, D. M.; Ten Dijke, P.; Sun, Z.

In: Cancer Research, Vol. 61, No. 5, 01.03.2001, p. 2112-2118.

Research output: Contribution to journalArticle

Hayes, SA, Zarnegar, M, Sharma, M, Yang, F, Peehl, DM, Ten Dijke, P & Sun, Z 2001, 'SMAD3 represses androgen receptor-mediated transcription', Cancer Research, vol. 61, no. 5, pp. 2112-2118.
Hayes SA, Zarnegar M, Sharma M, Yang F, Peehl DM, Ten Dijke P et al. SMAD3 represses androgen receptor-mediated transcription. Cancer Research. 2001 Mar 1;61(5):2112-2118.
Hayes, S. A. ; Zarnegar, M. ; Sharma, M. ; Yang, Fajun ; Peehl, D. M. ; Ten Dijke, P. ; Sun, Z. / SMAD3 represses androgen receptor-mediated transcription. In: Cancer Research. 2001 ; Vol. 61, No. 5. pp. 2112-2118.
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