Slug promotes survival during metastasis through suppression of puma-mediated apoptosis

Seaho Kim, Jiahong Yao, Kimita Suyama, Xia Qian, Bin Zhi Qian, Sanmay Bandyopadhyay, Olivier Loudig, Carlos De Leon-Rodriguez, Zhen Ni Zhou, Jeffrey E. Segall, Fernando Macian-Juan, Larry Norton, Rachel Hazan

Research output: Contribution to journalArticle

22 Citations (Scopus)

Abstract

Tumor cells must overcome apoptosis to survive throughout metastatic dissemination and distal organ colonization. Here, we show in the Polyoma Middle T mammary tumor model that N-cadherin (Cdh2) expression causes Slug (Snai2) upregulation, which in turn promotes carcinoma cell survival. Slug was dramatically upregulated in metastases relative to primary tumors. Consistent with a role in metastasis, Slug knockdown in carcinoma cells suppressed lung colonization by decreasing cell survival at metastatic sites, but had no effect on tumor cell invasion or extravasation. In support of this idea, Slug inhibition by shRNA sensitized tumor cells to apoptosis by DNA damage, resulting in caspase-3 and PARP cleavage. The prosurvival effect of Slug was found to be caused by direct repression of the proapoptotic gene, Puma (Bbc3), by Slug. Consistent with a pivotal role for a Slug-Puma axis in metastasis, inhibition of Puma by RNA interference in Slug-knockdown cells rescued lung colonization, whereas Puma overexpression in control tumor cells suppressed lung metastasis. The survival function of the Slug -Puma axis was confirmed in human breast cancer cells, where Slug knockdown increased Puma expression and inhibited lung colonization. This study demonstrates a pivotal role for Slug in carcinoma cell survival, implying that disruption of the Slug-Puma axis may impinge on the survival of metastatic cells.

Original languageEnglish (US)
Pages (from-to)3695-3706
Number of pages12
JournalCancer Research
Volume74
Issue number14
DOIs
StatePublished - Jul 15 2014

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Puma
Gastropoda
Apoptosis
Neoplasm Metastasis
Survival
Cell Survival
Lung
Neoplasms
Carcinoma
Breast Neoplasms
Cadherins
RNA Interference
Caspase 3
Small Interfering RNA
DNA Damage

ASJC Scopus subject areas

  • Cancer Research
  • Oncology

Cite this

Slug promotes survival during metastasis through suppression of puma-mediated apoptosis. / Kim, Seaho; Yao, Jiahong; Suyama, Kimita; Qian, Xia; Qian, Bin Zhi; Bandyopadhyay, Sanmay; Loudig, Olivier; De Leon-Rodriguez, Carlos; Zhou, Zhen Ni; Segall, Jeffrey E.; Macian-Juan, Fernando; Norton, Larry; Hazan, Rachel.

In: Cancer Research, Vol. 74, No. 14, 15.07.2014, p. 3695-3706.

Research output: Contribution to journalArticle

Kim, Seaho ; Yao, Jiahong ; Suyama, Kimita ; Qian, Xia ; Qian, Bin Zhi ; Bandyopadhyay, Sanmay ; Loudig, Olivier ; De Leon-Rodriguez, Carlos ; Zhou, Zhen Ni ; Segall, Jeffrey E. ; Macian-Juan, Fernando ; Norton, Larry ; Hazan, Rachel. / Slug promotes survival during metastasis through suppression of puma-mediated apoptosis. In: Cancer Research. 2014 ; Vol. 74, No. 14. pp. 3695-3706.
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