SLC19A3 encodes a second thiamine transporter ThTr2

Arun Rajgopal, Antoinette Edmondnson, I. David Goldman, Rongbao Zhao

Research output: Contribution to journalArticle

110 Scopus citations

Abstract

Recently, a new family of facilitative carriers has been cloned consisting of the reduced folate (SLC19A1) and the thiamine (SLC19A2) transporters. Despite a high level of sequence identity and similarity there is essentially no functional overlap between these carriers. The former transports folates and the latter thiamine. In this paper we describe the function of SLC19A3, another member of this transporter family most recently cloned, after transient transfection of the cDNA into HeLa cells. Uptake of [3H]thiamine, but not of methotrexate nor folic acid, was enhanced in SLC19A3 transfectants relative to vector control. Similarly, in the transfectants thiamine transport increased with an increase in pH with peak activity at pH ∼7.5. While [3H]thiamine uptake was markedly inhibited by nonlabeled thiamine it was not inhibited by several organic cations in 100-fold excess. Hence this carrier has a high degree of specificity for vitamin B1. The data indicate that SLC19A3 has the characteristics of SLC19A2 (ThTr1) and represents a second thiamine transporter (ThTr2) in this family of facilitative carriers.

Original languageEnglish (US)
Pages (from-to)175-178
Number of pages4
JournalBiochimica et Biophysica Acta - Molecular Basis of Disease
Volume1537
Issue number3
DOIs
StatePublished - Nov 29 2001

Keywords

  • SLC19A family
  • SLC19A3 function
  • Thiamine transport
  • Thiamine transporter

ASJC Scopus subject areas

  • Molecular Medicine
  • Molecular Biology

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