Six Innexins Contribute to Electrical Coupling of C. elegans Body-Wall Muscle

Ping Liu, Bojun Chen, Zeynep F. Altun, Maegan J. Gross, Alan Shan, Benjamin Schuman, David H. Hall, Zhao Wen Wang

Research output: Contribution to journalArticle

10 Citations (Scopus)

Abstract

C. elegans body-wall muscle cells are electrically coupled through gap junctions. Previous studies suggest that UNC-9 is an important, but not the only, innexin mediating the electrical coupling. Here we analyzed junctional current (Ij) for mutants of additional innexins to identify the remaining innexin(s) important to the coupling. The results suggest that a total of six innexins contribute to the coupling, including UNC-9, INX-1, INX-10, INX-11, INX-16, and INX-18. The Ij deficiency in each mutant was rescued completely by expressing the corresponding wild-type innexin specifically in muscle, suggesting that the innexins function cell-autonomously. Comparisons of Ij between various single, double, and triple mutants suggest that the six innexins probably form two distinct populations of gap junctions with one population consisting of UNC-9 and INX-18 and the other consisting of the remaining four innexins. Consistent with their roles in muscle electrical coupling, five of the six innexins showed punctate localization at muscle intercellular junctions when expressed as GFP- or epitope-tagged proteins, and muscle expression was detected for four of them when assessed by expressing GFP under the control of innexin promoters. The results may serve as a solid foundation for further explorations of structural and functional properties of gap junctions in C. elegans body-wall muscle.

Original languageEnglish (US)
Article numbere76877
JournalPLoS One
Volume8
Issue number10
DOIs
StatePublished - Oct 9 2013

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integument
Muscle
Gap Junctions
gap junctions
Muscles
muscles
mutants
Intercellular Junctions
Muscle Proteins
intercellular junctions
Muscle Cells
Population
Epitopes
myocytes
epitopes
functional properties
Cells
promoter regions
proteins
cells

ASJC Scopus subject areas

  • Agricultural and Biological Sciences(all)
  • Biochemistry, Genetics and Molecular Biology(all)
  • Medicine(all)

Cite this

Liu, P., Chen, B., Altun, Z. F., Gross, M. J., Shan, A., Schuman, B., ... Wang, Z. W. (2013). Six Innexins Contribute to Electrical Coupling of C. elegans Body-Wall Muscle. PLoS One, 8(10), [e76877]. https://doi.org/10.1371/journal.pone.0076877

Six Innexins Contribute to Electrical Coupling of C. elegans Body-Wall Muscle. / Liu, Ping; Chen, Bojun; Altun, Zeynep F.; Gross, Maegan J.; Shan, Alan; Schuman, Benjamin; Hall, David H.; Wang, Zhao Wen.

In: PLoS One, Vol. 8, No. 10, e76877, 09.10.2013.

Research output: Contribution to journalArticle

Liu, P, Chen, B, Altun, ZF, Gross, MJ, Shan, A, Schuman, B, Hall, DH & Wang, ZW 2013, 'Six Innexins Contribute to Electrical Coupling of C. elegans Body-Wall Muscle', PLoS One, vol. 8, no. 10, e76877. https://doi.org/10.1371/journal.pone.0076877
Liu P, Chen B, Altun ZF, Gross MJ, Shan A, Schuman B et al. Six Innexins Contribute to Electrical Coupling of C. elegans Body-Wall Muscle. PLoS One. 2013 Oct 9;8(10). e76877. https://doi.org/10.1371/journal.pone.0076877
Liu, Ping ; Chen, Bojun ; Altun, Zeynep F. ; Gross, Maegan J. ; Shan, Alan ; Schuman, Benjamin ; Hall, David H. ; Wang, Zhao Wen. / Six Innexins Contribute to Electrical Coupling of C. elegans Body-Wall Muscle. In: PLoS One. 2013 ; Vol. 8, No. 10.
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