Site-Specific Photo-Crosslinking Proteomics Reveal Regulation of IFITM3 Trafficking and Turnover by VCP/p97 ATPase

Xiaojun Wu, Jennifer S. Spence, Tandrila Das, Xiaoqiu Yuan, Chengjie Chen, Yuqing Zhang, Yumeng Li, Yanan Sun, Kartik Chandran, Howard C. Hang, Tao Peng

Research output: Contribution to journalArticle

Abstract

Interferon-induced transmembrane protein 3 (IFITM3) is a key interferon effector that broadly prevents infection by diverse viruses. However, the cellular factors that control IFITM3 homeostasis and antiviral activity have not been fully elucidated. Using site-specific photo-crosslinking and quantitative proteomic analysis, here we present the identification and functional characterization of VCP/p97 AAA-ATPase as a primary interaction partner of IFITM3. We show that IFITM3 ubiquitination at lysine 24 is crucial for VCP binding, trafficking, turnover, and engagement with incoming virus particles. Consistently, pharmacological inhibition of VCP/p97 ATPase activity leads to defective IFITM3 lysosomal sorting, turnover, and co-trafficking with virus particles. Our results showcase the utility of site-specific protein photo-crosslinking in mammalian cells and reveal VCP/p97 as a key cellular factor involved in IFITM3 trafficking and homeostasis.

Original languageEnglish (US)
Pages (from-to)571-585.e6
JournalCell Chemical Biology
Volume27
Issue number5
DOIs
StatePublished - May 21 2020

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Keywords

  • IFITM3
  • VCP/p97
  • chemical proteomics
  • photo-crosslinking
  • protein-protein interaction
  • unnatural amino acid

ASJC Scopus subject areas

  • Biochemistry
  • Molecular Medicine
  • Molecular Biology
  • Pharmacology
  • Drug Discovery
  • Clinical Biochemistry

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