Site of binding of mouse IgG(2b) to the Fc receptor on mouse macrophages

B. Diamond, B. K. Birshtein, Matthew D. Scharff

Research output: Contribution to journalArticle

17 Citations (Scopus)

Abstract

Three mouse immunoglobulins with altered heavy chains have been used to study the specificity of the mouse IgG(2b) Fc receptor on mouse macrophages. These immunoglobulins were synthesized by variant clones derived from the MPC 11, IgG(2b)-producing mouse myeloma cell line. One variant, whose Fc seems identical to that of an IgG(2a) myeloma protein of known sequence, binds specifically to the IgG(2a) Fc receptor. A second variant, which makes a short heavy chain lacking the C(H)3 domain, binds specifically to the IgG(2b) Fc receptor. The third variant makes a hybrid IgG(2b)-IgG(2a) heavy chain whose C(H)3 domain is entirely IgG(2a)-like and binds to both IgG(2a) and IgG(2b) Fc receptors. These data suggest that the binding of mouse IgG(2b) immunoglobulins to the mouse macrophage Fc receptor involves a site within the C(H)2 domain and indicate that immunoglobulins with altered heavy chains are a useful tool to probe Fc receptors.

Original languageEnglish (US)
Pages (from-to)721-726
Number of pages6
JournalJournal of Experimental Medicine
Volume150
Issue number3
StatePublished - 1979

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Fc Receptors
Immunoglobulin G
Macrophages
Binding Sites
Immunoglobulins
Myeloma Proteins
Clone Cells

ASJC Scopus subject areas

  • Immunology

Cite this

Site of binding of mouse IgG(2b) to the Fc receptor on mouse macrophages. / Diamond, B.; Birshtein, B. K.; Scharff, Matthew D.

In: Journal of Experimental Medicine, Vol. 150, No. 3, 1979, p. 721-726.

Research output: Contribution to journalArticle

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