Site-directed Mutagenesis in the Fingers Subdomain of HIV-1 Reverse Transcriptase Reveals a Specific Role for the β3-β4 Hairpin Loop in dNTP Selection

Scott J. Garforth, Tae Woo Kim, Michael A. Parniak, Eric T. Kool, Vinayaka R. Prasad

Research output: Contribution to journalArticle

18 Scopus citations

Abstract

HIV-1 reverse transcriptase shares the key features of high fidelity polymerases, such as a closed architecture of the active site, but displays a level of fidelity that is intermediate to that of high fidelity, replicative polymerases and low fidelity translesion synthesis (TLS) polymerases. The β3-β4 loop of the HIV-1 RT fingers subdomain makes transient contacts with the dNTP and template base. To investigate the role of active site architecture in HIV-1 RT fidelity, we truncated the β3-β4 loop, eliminating contact between Lys65 and the γ-phosphate of dNTP. The mutant, in a manner reminiscent of TLS polymerases, was only able to incorporate a nucleotide that was capable of base-pairing with the template nucleotide, but not a nucleotide shape-analog incapable of Watson-Crick hydrogen bonding. Unexpectedly, however, the deletion mutant differed from the TLS polymerases in that it displayed an increased fidelity. The increased fidelity was associated with reduced dNTP binding affinity as measured using the dead end complex formation. In an effort to delineate the specific amino acid residue in the deleted segment responsible for this phenotype, we examined the K65 residue. Two substitution mutants, K65R and K65A were studied. The K65A mutant behaved similarly to the deletion mutant displaying dependence on Watson-Crick hydrogen bonding, increased fidelity and reduced dNTP-binding, while the K65R was more akin to wild-type enzyme. These results underscore the key role of the K65 residue in the phenotype observed in the deletion mutant. Based on the well-known electrostatic interaction between K65 and the γ-phosphate moiety of incoming dNTP substrate in the ternary complex structure of HIV-1 RT, we conclude that non-discriminatory interactions between β3-β4 loop and the dNTP in wild-type HIV-1 RT help lower dNTP selectivity. Our results show that the fidelity of dNTP insertion is influenced by protein interactions with the triphosphate moiety.

Original languageEnglish (US)
Pages (from-to)38-49
Number of pages12
JournalJournal of Molecular Biology
Volume365
Issue number1
DOIs
Publication statusPublished - Jan 5 2007

    Fingerprint

Keywords

  • DNA replication
  • K65R
  • RT fidelity
  • dNTP selection
  • steric effects

ASJC Scopus subject areas

  • Structural Biology
  • Molecular Biology

Cite this