Single-Stranded DNA as a cleavable linker for bioorthogonal click chemistry-based proteomics

Tianqing Zheng, Hao Jiang, Peng Wu

Research output: Contribution to journalArticle

17 Citations (Scopus)

Abstract

In this communication, we report a new class of cleavable linker based on automatically synthesized, single-stranded DNAs. We incorporated a DNA oligo into an azide-functionalized biotin (biotin-DNA-N3) and used the probe to enrich for alkyne-tagged glycoproteins from mammalian cell lysates. Highly efficient and selective release of the captured proteins from streptavidin agarose resins was achieved using DNase treatment under very mild conditions. A total of 36 sialylated glycoproteins were identified from the lysates of HL60 cells, an acute human promyeloid leukemia cell line. These sialylated glycoproteins were involved in many different biological processes ranging from glycan biosynthesis to cell adhesion events.

Original languageEnglish (US)
Pages (from-to)859-864
Number of pages6
JournalBioconjugate Chemistry
Volume24
Issue number6
DOIs
StatePublished - Jun 19 2013

Fingerprint

Click Chemistry
Glycoproteins
Single-Stranded DNA
Proteomics
DNA
Biotin
Cells
Biological Phenomena
Alkynes
Azides
Deoxyribonucleases
HL-60 Cells
Biosynthesis
Cell adhesion
Cell Adhesion
Polysaccharides
Leukemia
Resins
Proteins
Cell Line

ASJC Scopus subject areas

  • Biotechnology
  • Bioengineering
  • Organic Chemistry
  • Pharmaceutical Science
  • Biomedical Engineering
  • Pharmacology

Cite this

Single-Stranded DNA as a cleavable linker for bioorthogonal click chemistry-based proteomics. / Zheng, Tianqing; Jiang, Hao; Wu, Peng.

In: Bioconjugate Chemistry, Vol. 24, No. 6, 19.06.2013, p. 859-864.

Research output: Contribution to journalArticle

Zheng, Tianqing ; Jiang, Hao ; Wu, Peng. / Single-Stranded DNA as a cleavable linker for bioorthogonal click chemistry-based proteomics. In: Bioconjugate Chemistry. 2013 ; Vol. 24, No. 6. pp. 859-864.
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