Single mRNAs visualized by ultrastructural in situ hybridization are principally localized at actin filament intersections in fibroblasts

Gary J. Bassell, Christine M. Powers, Krishan L. Taneja, Robert H. Singer

Research output: Contribution to journalArticle

158 Citations (Scopus)

Abstract

Considerable evidence indicates that mRNA associates with structural filaments in the cell (cytoskeleton). This relationship would be an important mechanism to effect mRNA sorting since specific mRNAs could be sequestered at sites within the cell. In addition, it can provide a mechanism for spatial regulation of mRNA expression. However, the precise structural interactions between mRNA and the cytoskeleton have yet to be defined. An objective of this work was to visualize 'individual' poly(A) mRNA molecules in situ by electron microscopy to identify their relationship to individual filaments. Poly(A) RNA and filaments were identified simultaneously using antibodies to detect hybridized probe and filaments or actin-binding proteins. In human fibroblasts, most of the poly(A) mRNA (72%) was localized within 5 nm of orthogonal networks of F-actin filaments. Poly(A) mRNA also colocalized with vimentin filaments (29%) and microtubules (<10%). The sites of mRNA localization were predominantly at filament intersections. The majority of poly(A) mRNA and polysomes colocalized with the actin crosslinking proteins, filamin, and α-actinin, and the elongation factor, EF-1α (actin-binding protein; ABP-50). Evidence that intersections contained single mRNA molecules was provided by using a labeled oligo dT probe to prime the synthesis of cDNA in situ using reverse transcriptase. Both the poly(A) and cis sequences of the same mRNA molecule could then be visualized independently. We propose that the cytoskeletal intersection is a mRNA receptor and serves as a 'microdomain' where mRNA is attached and functionally expressed.

Original languageEnglish (US)
Pages (from-to)863-876
Number of pages14
JournalJournal of Cell Biology
Volume126
Issue number4
DOIs
StatePublished - Aug 1994
Externally publishedYes

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Actin Cytoskeleton
In Situ Hybridization
Fibroblasts
Messenger RNA
Peptide Elongation Factor 1
Microfilament Proteins
Cytoskeleton
Actins
RNA Transport
Filamins
Actinin
Poly A
Polyribosomes
RNA-Directed DNA Polymerase
Vimentin
Microtubules
Electron Microscopy
Complementary DNA

ASJC Scopus subject areas

  • Cell Biology

Cite this

Single mRNAs visualized by ultrastructural in situ hybridization are principally localized at actin filament intersections in fibroblasts. / Bassell, Gary J.; Powers, Christine M.; Taneja, Krishan L.; Singer, Robert H.

In: Journal of Cell Biology, Vol. 126, No. 4, 08.1994, p. 863-876.

Research output: Contribution to journalArticle

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