Single mRNA Tracking in Live Cells

Hye Yoon Park, Adina R. Buxbaum, Robert H. Singer

Research output: Chapter in Book/Report/Conference proceedingChapter

50 Scopus citations

Abstract

Asymmetric distribution of mRNA is a prevalent phenomenon observed in diverse cell types. The posttranscriptional movement and localization of mRNA provides an important mechanism to target certain proteins to specific cytoplasmic regions of their function. Recent technical advances have enabled real-time visualization of single mRNA molecules in living cells. Studies analyzing the motion of individual mRNAs have shed light on the complex RNA transport system. This chapter presents an overview of general approaches for single particle tracking and some methodologies that are used for single mRNA detection.

Original languageEnglish (US)
Title of host publicationMethods in Enzymology
PublisherAcademic Press Inc.
Pages387-406
Number of pages20
DOIs
StatePublished - Jan 2010

Publication series

NameMethods in Enzymology
Volume472
ISSN (Print)0076-6879
ISSN (Electronic)1557-7988

ASJC Scopus subject areas

  • Biochemistry
  • Molecular Biology

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