Single mRNA tracking in live cells.

Hye Yoon Park, Adina R. Buxbaum, Robert H. Singer

Research output: Contribution to journalArticle

48 Scopus citations

Abstract

Asymmetric distribution of mRNA is a prevalent phenomenon observed in diverse cell types. The posttranscriptional movement and localization of mRNA provides an important mechanism to target certain proteins to specific cytoplasmic regions of their function. Recent technical advances have enabled real-time visualization of single mRNA molecules in living cells. Studies analyzing the motion of individual mRNAs have shed light on the complex RNA transport system. This chapter presents an overview of general approaches for single particle tracking and some methodologies that are used for single mRNA detection.

Original languageEnglish (US)
Pages (from-to)387-406
Number of pages20
JournalMethods in enzymology
Volume472
StatePublished - 2010

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ASJC Scopus subject areas

  • Biochemistry
  • Molecular Biology

Cite this

Park, H. Y., Buxbaum, A. R., & Singer, R. H. (2010). Single mRNA tracking in live cells. Methods in enzymology, 472, 387-406.