TY - JOUR
T1 - Single-molecule fluorescence spectroscopy and microscopy of biomolecular motors
AU - Peterman, Erwin J.G.
AU - Sosa, Hernando
AU - Moerner, W. E.
PY - 2004
Y1 - 2004
N2 - The methods of single-molecule fluorescence spectroscopy and microscopy have been recently utilized to explore the mechanism of action of several members of the kinesin and myosin biomolecular motor protein families. Whereas ensemble averaging is removed in single-molecule studies, heterogeneity in the behavior of individual motors can be directly observed, without synchronization. Observation of translocation by individual copies of motor proteins allows analysis of step size, rate, pausing, and other statistical properties of the process. Polarization microscopy as a function of nucleotide state has been particularly useful in revealing new and highly rotationally mobile forms of particular motors. These experiments complement X-ray and biochemical studies and provide a detailed view into the local dynamical behavior of motor proteins.
AB - The methods of single-molecule fluorescence spectroscopy and microscopy have been recently utilized to explore the mechanism of action of several members of the kinesin and myosin biomolecular motor protein families. Whereas ensemble averaging is removed in single-molecule studies, heterogeneity in the behavior of individual motors can be directly observed, without synchronization. Observation of translocation by individual copies of motor proteins allows analysis of step size, rate, pausing, and other statistical properties of the process. Polarization microscopy as a function of nucleotide state has been particularly useful in revealing new and highly rotationally mobile forms of particular motors. These experiments complement X-ray and biochemical studies and provide a detailed view into the local dynamical behavior of motor proteins.
KW - Kinesin
KW - Molecular motor proteins
KW - Myosin
KW - Single biomolecule detection
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U2 - 10.1146/annurev.physchem.55.091602.094340
DO - 10.1146/annurev.physchem.55.091602.094340
M3 - Article
C2 - 15117248
AN - SCOPUS:3342942688
SN - 0066-426X
VL - 55
SP - 79
EP - 96
JO - Annual Review of Physical Chemistry
JF - Annual Review of Physical Chemistry
ER -