Single-molecule analysis of gene expression using two-color RNA labeling in live yeast

Sami Hocine, Pascal Raymond, Daniel Zenklusen, Jeffrey A. Chao, Robert H. Singer

Research output: Contribution to journalArticle

158 Citations (Scopus)

Abstract

Live-cell imaging of mRNA yields important insights into gene expression, but it has generally been limited to the labeling of one RNA species and has never been used to count single mRNAs over time in yeast. We demonstrate a two-color imaging system with single-molecule resolution using MS2 and PP7 RNA labeling. We use this methodology to measure intrinsic noise in mRNA levels and RNA polymerase II kinetics at a single gene.

Original languageEnglish (US)
Pages (from-to)119-121
Number of pages3
JournalNature Methods
Volume10
Issue number2
DOIs
StatePublished - Feb 2013

Fingerprint

Gene expression
Yeast
Labeling
Color
Yeasts
RNA
Gene Expression
Messenger RNA
Molecules
RNA Polymerase II
Imaging systems
Noise
Genes
Imaging techniques
Kinetics

ASJC Scopus subject areas

  • Biotechnology
  • Molecular Biology
  • Biochemistry
  • Cell Biology

Cite this

Single-molecule analysis of gene expression using two-color RNA labeling in live yeast. / Hocine, Sami; Raymond, Pascal; Zenklusen, Daniel; Chao, Jeffrey A.; Singer, Robert H.

In: Nature Methods, Vol. 10, No. 2, 02.2013, p. 119-121.

Research output: Contribution to journalArticle

Hocine, Sami ; Raymond, Pascal ; Zenklusen, Daniel ; Chao, Jeffrey A. ; Singer, Robert H. / Single-molecule analysis of gene expression using two-color RNA labeling in live yeast. In: Nature Methods. 2013 ; Vol. 10, No. 2. pp. 119-121.
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