Single-molecule analysis of gene expression using two-color RNA labeling in live yeast

Sami Hocine, Pascal Raymond, Daniel Zenklusen, Jeffrey A. Chao, Robert H. Singer

Research output: Contribution to journalArticle

160 Scopus citations

Abstract

Live-cell imaging of mRNA yields important insights into gene expression, but it has generally been limited to the labeling of one RNA species and has never been used to count single mRNAs over time in yeast. We demonstrate a two-color imaging system with single-molecule resolution using MS2 and PP7 RNA labeling. We use this methodology to measure intrinsic noise in mRNA levels and RNA polymerase II kinetics at a single gene.

Original languageEnglish (US)
Pages (from-to)119-121
Number of pages3
JournalNature Methods
Volume10
Issue number2
DOIs
StatePublished - Feb 1 2013

ASJC Scopus subject areas

  • Biotechnology
  • Biochemistry
  • Molecular Biology
  • Cell Biology

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